Molecular marker

Fine mapping and characterization of the or gene in Chinese cabbage (Brassica rapa L. ssp pekinensis)

C. L. Zou, Zheng, Y., Wang, P., Zhang, X., Wang, Y. - H., Liu, Z. Y., Feng, H., Zou, C. L., Zheng, Y., Wang, P., Zhang, X., Wang, Y. - H., Liu, Z. Y., and Feng, H., Fine mapping and characterization of the or gene in Chinese cabbage (Brassica rapa L. ssp pekinensis), vol. 15, p. -, 2016.

Orange inner leaves/heads is a qualitative trait in Chinese cabbage that is controlled by a single recessive gene. Orange head Chinese cabbage contain more carotenoids than its white head counterpart; hence, this trait is of interest to both researchers and consumers. In this study, we selected the orange head Chinese cabbage line 07A163 and the white head Chinese cabbage line Chiifu as test materials.

Breeding of a target genotype variety based on identified chalkiness marker-QTL associations in rice (Oryza sativa L.)

X. Liu, Du, Y. R., Li, X. H., Li, X. L., Yang, W. Q., and Wang, Y., Breeding of a target genotype variety based on identified chalkiness marker-QTL associations in rice (Oryza sativa L.), vol. 14, pp. 12894-12902, 2015.

The aim of this study was to breed a target genotype variety based on the identified chalkiness marker-QTL (quantitative trait locus) associations in rice. First, a permanent mapping population of rice that consisted of 525 recombinant inbred lines (RILs), which were derived from Zhenshan 97/Minghui 63, was used to identify QTLs with additive effects for rice quantitative traits and percentage of grain chalkiness (PGC). Subsequently, based on the identified QTLs in rice, the molecular marker 68923-PGC was selected to screen the low chalkiness rice line.

Identification and evaluation of polymorphisms in FABP3 and FABP4 in beef cattle

I. M. Z. Blecha, Siqueira, F., Ferreira, A. B. R., Feijó, G. L. D., Júnior, R. A. A. Torres, Medeiros, S. R., Sousa, I. I., Santiago, G. G., and Ferraz, A. L. J., Identification and evaluation of polymorphisms in FABP3 and FABP4 in beef cattle, vol. 14, pp. 16353-16363, 2015.

Single nucleotide polymorphisms (SNPs) were screened in FABP3 and FABP4 by automatic sequencing of pools of DNA from crossbred animals whose phenotypes belonged to the upper and lower extremes for back fat and marbling, as well as of a pool of DNA from sires used for crossbreeding. Five SNPs were identified in FABP3 and another nine SNPs were identified in FAPB4. Of these, only one SNP had no previous registry in the SNAP database (dbSNP).

Marker-assisted selection of Fusarium wilt-resistant and gynoecious melon (Cucumis melo L.)

P. Gao, Liu, S., Zhu, Q. L., and Luan, F. S., Marker-assisted selection of Fusarium wilt-resistant and gynoecious melon (Cucumis melo L.), vol. 14, pp. 16255-16264, 2015.

In this study, molecular markers were designed based on the sex determination genes ACS7 (A) and WIP1 (G) and the domain in the Fusarium oxysporum-resistant gene Fom-2 (F) in order to achieve selection of F. oxysporum-resistant gynoecious melon plants. Markers of A and F are cleaved amplified polymorphic sequences that distinguish alleles according to restriction analysis.

Genetic variation in Rhodomyrtus tomentosa (Kemunting) populations from Malaysia as revealed by inter-simple sequence repeat markers

T. S. Hue, Abdullah, T. L., Abdullah, N. A. P., and Sinniah, U. R., Genetic variation in Rhodomyrtus tomentosa (Kemunting) populations from Malaysia as revealed by inter-simple sequence repeat markers, vol. 14, pp. 16827-16839, 2015.

Kemunting (Rhodomyrtus tomentosa) from the Myrtaceae family, is native to Malaysia. It is widely used in traditional medicine to treat various illnesses and possesses significant antibacterial properties. In addition, it has great potential as ornamental in landscape design. Genetic variability studies are important for the rational management and conservation of genetic material. In the present study, inter-simple sequence repeat markers were used to assess the genetic diversity of 18 R. tomentosa populations collected from ten states of Peninsular Malaysia.

Genetic characterization of red-colored heartwood genotypes of Chinese fir using simple sequence repeat (SSR) markers

H. J. Duan, Hu, R. Y., Wu, B., Chen, D. X., Huang, K. Y., Dai, J., Chen, Q., Wei, Z. C., Cao, S., Sun, Y. H., and Li, Y., Genetic characterization of red-colored heartwood genotypes of Chinese fir using simple sequence repeat (SSR) markers, vol. 14, pp. 18552-18561, 2015.

The present study investigated the genetic characterization of red-colored heartwood Chinese fir [Cunninghamia lanceolata (Lamb.) Hook.] in Guangxi using 21 simple sequence repeat (SSR) markers and analyzes of the genetic variation (N = 149) in samples obtained from five sites in Guangxi Province, China. The number of different alleles and the Shannon’s information index per locus ranged from 3 to 12 and from 0.398 to 2.258 with average values of 6 and 1.211, respectively, indicating moderate levels of genetic diversity within this germplasm collection.

Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers

Á. M. Vidal, Vieira, L. J., Ferreira, C. F., Souza, F. V. D., Souza, A. S., and Ledo, C. A. S., Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers, vol. 14, pp. 7759-7770, 2015.

Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro.

Pages

Subscribe to Molecular marker