Molecular characterization

Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers

G. A. Luz, Gomes, S. O., Neto, R. B. Araújo, Nascimento, M. S. C. B., and Lima, P. S. C., Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers, vol. 14, pp. 15242-15248, 2015.

Cratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence.

Molecular characterization of Babesia vogeli in dogs from Belém, northern Brazil

P. H. G. Moraes, Rufino, C. P., Baraúna, A. R. F., Reis, T., Agnol, L. T. D., Meneses, A. M. C., Aguiar, D. C. F., Nunes, M. R. T., and Gonçalves, E. C., Molecular characterization of Babesia vogeli in dogs from Belém, northern Brazil, vol. 14, pp. 16364-16371, 2015.

Babesiosis is an infectious hemolytic disease that occurs worldwide, and is caused by a protozoan of the Babesia genus (Apicomplexa). Little is known about this genus; therefore, this study conducted a molecular characterization of Babesia spp in naturally infected dogs in northern Brazil. Blood samples were collected from 172 dogs from metropolitan Belém, and screened for Babesia spp using semi-nested polymerase chain reactions. Subsequently, 18S rDNA gene fragments were amplified and sequenced.

Evaluation of microsatellite loci from libraries derived from the wild diploid 'Calcutta 4' and 'Ouro' banana cultivars

P. R. O. Silva, Jesus, O. N. J., Creste, S., Figueira, A., Amorim, E. P., and Ferreira, C. F., Evaluation of microsatellite loci from libraries derived from the wild diploid 'Calcutta 4' and 'Ouro' banana cultivars, vol. 14, pp. 11410-11428, 2015.

Microsatellite markers have been widely used in the quantification of genetic variability and for genetic breeding in Musa spp. The objective of the present study was to evaluate the discriminatory power of microsatellite markers derived from ‘Calcutta 4’ and ‘Ouro’ genomic libraries, and to analyze the genetic variability among 30 banana accessions. Thirty-eight markers were used: 15 from the ‘Ouro’ library and 23 from the ‘Calcutta 4’ library.

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