Mitochondrial control region

Comparison of DNA extraction methods for polymerase chain reaction amplification of guanaco (Lama guanicoe) fecal DNA samples

M. I. Espinosa, Bertin, A., Squeo, F. A., Cortés, A., and Gouin, N., “Comparison of DNA extraction methods for polymerase chain reaction amplification of guanaco (Lama guanicoe) fecal DNA samples”, vol. 14, pp. 400-406, 2015.

Feces-based population genetic studies have become increasingly popular. However, polymerase chain reaction (PCR) amplification rates from fecal material vary depending on the species, populations, loci, and extraction protocols. Here, we assessed the PCR amplification success of three microsatellite markers and a segment of the mitochondrial control region of DNA extracted from field-collected feces of guanaco (Lama guanicoe) using two protocols - Qiagen DNA Stool Kit and 2 cetyltrimethylammonium bromide/phenol:chloroform:isoamyl alcohol (2CTAB/PCI) method.

Genetic polymorphisms of LPL and HL and their association with the performance of Chinese sturgeons fed a formulated diet

Y. He, Shen, D., Liang, X. F., Lu, R. H., and Xiao, H., “Genetic polymorphisms of LPL and HL and their association with the performance of Chinese sturgeons fed a formulated diet”, vol. 12. pp. 4559-4566, 2013.

It is very important to investigate the reasons for the large individual differences in individual performance of food acceptance when using formulated diets for the successful culture of larvae and juveniles of the Chinese sturgeon Acipenser sinensis. Genetic differences of the mitochondrial control region were investigated by direct sequencing in two groups of Chinese sturgeon, which were apt to accept or refuse formulated diets. Among 968-bp sequences, 111 variable sites were identified.

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