Minisequencing

Optimization of a multiplex minisequencing protocol for population studies and medical genetics

C. M. B. Carvalho and Pena, S. D. J., Optimization of a multiplex minisequencing protocol for population studies and medical genetics, vol. 4, pp. 115-125, 2005.

Several technologically sophisticated high-throughput techniques have been recently developed for the study of human single nucleotide polymorphisms and the diagnosis of point mutations in human diseases. However, there is also a need for simple and inexpensive techniques suitable for clinical services and small research laboratories. Minisequencing meets the latter requirements. It is simple, non-radioactive and can be easily multiplexed by adding oligonucleotide tails of increasing size to the sequencing oligonucleotide primers.

Subscribe to Minisequencing