Microsatellites

Genetic analysis of the Venezuelan Criollo horse

E. G. Cothran, Canelon, J. L., Luis, C., Conant, E., and Juras, R., “Genetic analysis of the Venezuelan Criollo horse”, vol. 10, pp. 2394-2403, 2011.

Various horse populations in the Americas have an origin in Spain; they are remnants of the first livestock introduced to the continent early in the colonial period (16th and 17th centuries). We evaluated genetic variability within the Venezuelan Criollo horse and its relationship with other horse breeds. We observed high levels of genetic diversity within the Criollo breed. Significant population differentiation was observed between all South American breeds.

Eight polymorphic microsatellite markers for the spotted babylon, Babylonia areolata (Buccinidae)

Y. Wang, Lu, H., Zheng, J., Long, K., Shi, Y. H., Gu, Z. F., and Wang, A. M., “Eight polymorphic microsatellite markers for the spotted babylon, Babylonia areolata (Buccinidae)”, vol. 10, pp. 3230-3235, 2011.

The spotted babylon, Babylonia areolata, is one of the most extensively cultured marine mollusks in southeast Asia. Eight polymorphic microsatellite markers were developed for this species, from a microsatellite-enriched library. These markers, characterized in 32 individuals from a hatchery population, were polymorphic, with allele numbers ranging from 6 to 18 per locus, expected and observed heterozygosities ranging from 0.68 to 0.94 and 0.56 to 0.81, respectively.

Genetic diversity in the Pantaneiro horse breed assessed using microsatellite DNA markers

E. H. Giacomoni, Fernández-Stolz, G. P., and Freitas, T. R. O., “Genetic diversity in the Pantaneiro horse breed assessed using microsatellite DNA markers”, vol. 7, pp. 261-270, 2008.

The genetic variability for a sample of 227 animals from three populations of Pantaneiro horses was estimated using data from 10 microsatellite loci. The number of alleles and the proportion of heterozygosity indicated high variability. A total of 91 alleles were found, with a significantly high mean number of alleles. The mean polymorphic information content was 0.7 and the paternity exclusion probability was 99.3%. The inbreeding coefficient (Fis) was low for the three populations: Ipiranga (FIS = 0.147), Nova Esperança (Fis = 0.094) and Promissão (Fis = 0.108).

Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites

I. G. Hamoy, Santos, E. J. M., and Santos, S. E. B., “Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites”, vol. 7, pp. 29-32, 2008.

The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population.

Characterization of the resistance gradient of cacao genotypes (F2 Sca6 x ICS1) to black pod disease using phytopathometric, genetic and molecular approaches

E. S. L. Santos, “Characterization of the resistance gradient of cacao genotypes (F2 Sca6 x ICS1) to black pod disease using phytopathometric, genetic and molecular approaches”, vol. 8. pp. 1307-1308, 2009.

Allele frequencies and genetic diversity in two groups of wild tufted capuchin monkeys (Cebus apella nigritus) living in an urban forest fragment

J. Margareth Amaral, Simões, A. Luiz, and De Jong, D., “Allele frequencies and genetic diversity in two groups of wild tufted capuchin monkeys (Cebus apella nigritus) living in an urban forest fragment”, vol. 4, pp. 832-838, 2005.

There have been numerous studies genetically characterizing Old World Primates using microsatellites. However, few studies have been made of New World species and none on free-ranging Cebus apella, even though it is probably the most widely distributed species of monkey in the New World. The paucity of studies is due, in part, to the lack of polymorphisms described for this species.

Determination of ancestral proportions in synthetic bovine breeds using commonly employed microsatellite markers

H. M. S. Bicalho, Pimenta, C. G., Mendes, I. K. P., Pena, H. B., Queiroz, E. M., and Pena, S. D. J., “Determination of ancestral proportions in synthetic bovine breeds using commonly employed microsatellite markers”, vol. 5, pp. 432-437, 2006.

The International Society of Animal Genetics (ISAG) has chosen nine microsatellites (international marker set) as a standard that should be included in all cattle parentage studies. They are BM1824, BM2113, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH10, and ETH225. We decided to ascertain whether this microsatellite set could be used to determine ancestral proportions in individual animals of synthetic breeds produced by crossing zebu and taurine cattle.

A combination of five short tandem repeats of chromosome 15 significantly improves the identification of Prader-Willi syndrome etiology in the Argentinean population

H. V. Aráoz, Torrado, M., Barreiro, C., and Chertkoff, L., “A combination of five short tandem repeats of chromosome 15 significantly improves the identification of Prader-Willi syndrome etiology in the Argentinean population”, vol. 5, pp. 390-398, 2006.

Prader-Willi syndrome (PWS) is a multisystemic disorder caused by the loss of expression of paternally transcribed genes in the PWS critical region of chromosome 15. Various molecular mechanisms are known to lead to PWS: deletion 15q11-q13 (75% of cases), maternal uniparental disomy (matUPD15) (23%) and imprinting defects (2%). FISH and microsatellite analysis are required to establish the molecular etiology, which is essential for appropriate genetic counseling and care management.

Comparison of simple sequence repeats in 19 Archaea

S. Trivedi, “Comparison of simple sequence repeats in 19 Archaea”, vol. 5, pp. 741-772, 2006.

All organisms that have been studied until now have been found to have differential distribution of simple sequence repeats (SSRs), with more SSRs in intergenic than in coding sequences. SSR distribution was investigated in Archaea genomes where complete chromosome sequences of 19 Archaea were analyzed with the program SPUTNIK to find di- to penta-nucleotide repeats. The number of repeats was determined for the complete chromosome sequences and for the coding and non-coding sequences.

Temporal genetic differentiation in cultured and natural beds of the brown mussel Perna perna (Mytilidae)

K. T. Appio and Weber, L. I., “Temporal genetic differentiation in cultured and natural beds of the brown mussel Perna perna (Mytilidae)”, vol. 6, pp. 127-136, 2007.

Perna perna is the most important cultivated mussel of Santa Catarina, Brazil, sustaining an important economic input for many local families. Natural stocks of P. perna are depleted by the extraction of adults and seeds for consumption and culture. The aim of the present study was to use the microsatellite locus pms-2 to study the variation of the genetic composition and diversity between natural and cultured stocks in samples of 2001 and 2005 from Penha, Santa Catarina.

Pages

Subscribe to Microsatellites