Microsatellites

Isolation and characterization of novel microsatellite loci in the wrinkled frog

D. Y. Kim, Kim, D., Park, S. K., Lee, H., Suk, H. Y., Min, M. S., Kim, D. Y., Kim, D., Park, S. K., Lee, H., Suk, H. Y., Min, M. S., Kim, D. Y., Kim, D., Park, S. K., Lee, H., Suk, H. Y., and Min, M. S., Isolation and characterization of novel microsatellite loci in the wrinkled frog, vol. 15, p. -, 2016.

The wrinkled frog, Rana rugosa, is a species complex that inhabits plains and mountains near freshwater bodies throughout East Asia, encompassing China, Korea, Japan, and the Russian Primorye region. Although extensive efforts are required to estimate cryptic diversity in the R. rugosa complex, no specifically designed microsatellite loci are available. Here, novel and polymorphic microsatellites were isolated based on the construction of a microsatellite-enriched library and characterized using R. rugosa specimens collected on the Korean Peninsula.

Development of novel chloroplast microsatellite markers for Ginkgo biloba

M. Xu, Xu, L. A., Cao, F. L., Zhang, H. J., and Yu, F. X., Development of novel chloroplast microsatellite markers for Ginkgo biloba, vol. 14, pp. 7715-7720, 2015.

Ginkgo biloba is considered to be a living fossil that can be used to understand the ancient evolutionary history of gymnosperms, but little attention has been given to the study of its population genetics, molecular phylogeography, and genetic resources assessment. Chloro­plast simple sequence repeat (cpSSR) markers are powerful tools for genetic studies of plants. In this study, a total of 30 perfect cpSSRs of Ginkgo were identified and characterized, including di-, tri, tetra-, penta-, and hexanucleotide repeats.

Isolation and characterization of novel polymorphic microsatellite loci in large yellow croaker (Larimichthys crocea)

X. Q. Wang, Zeng, D., Ma, X., Xiong, G., Wang, Z. Y., and Sakamoto, T., Isolation and characterization of novel polymorphic microsatellite loci in large yellow croaker (Larimichthys crocea), vol. 14, pp. 9437-9440, 2015.

The large yellow croaker (Larimichthys crocea) is one of the largest marine net-cage cultured species in the oceans around China. In the present study, we isolated and characterized 13 polymorphic microsatellite markers from genomic libraries of L. crocea. Loci were screened for 10 wild specimens from 2 sites in southeast of China. All loci were polymorphic. The number of alleles per locus ranged from 2 to 21. The expected heterozygosity ranged from 0.233 to 0.838 and observed heterozygosity ranged from 0.527 to 0.935.

Selection of an effective microsatellite marker system for genetic control and analysis of gerbil populations in China

X. Y. Du, Li, W., Sa, X. Y., Li, C. L., Lu, J., Wang, Y. Z., and Chen, Z. W., Selection of an effective microsatellite marker system for genetic control and analysis of gerbil populations in China, vol. 14, pp. 11030-11042, 2015.

Although gerbils have been widely used in many areas of biological research over many years, there is currently no effective genetic quality control system available. In the present study, we sought to establish a microsatellite marker system for quality control and con­ducted an optimized analysis of 137 microsatellite loci in two labora­tory gerbil populations and one wild population.

Evaluation of microsatellite loci from libraries derived from the wild diploid 'Calcutta 4' and 'Ouro' banana cultivars

P. R. O. Silva, Jesus, O. N. J., Creste, S., Figueira, A., Amorim, E. P., and Ferreira, C. F., Evaluation of microsatellite loci from libraries derived from the wild diploid 'Calcutta 4' and 'Ouro' banana cultivars, vol. 14, pp. 11410-11428, 2015.

Microsatellite markers have been widely used in the quantification of genetic variability and for genetic breeding in Musa spp. The objective of the present study was to evaluate the discriminatory power of microsatellite markers derived from ‘Calcutta 4’ and ‘Ouro’ genomic libraries, and to analyze the genetic variability among 30 banana accessions. Thirty-eight markers were used: 15 from the ‘Ouro’ library and 23 from the ‘Calcutta 4’ library.

Subscribe to Microsatellites