Microsatellites

Development of polymorphic microsatellite loci in Odontobutis obscura using Illumina paired-end sequencing and the test of cross-species amplification

H. - G. Bae, An, J., Jun, J., Kweon, S. - M., Kim, S., Yu, J. - N., Kim, B. - J., Sakai, H., Lee, Y. J., Suk, H. Y., Bae, H. - G., An, J., Jun, J., Kweon, S. - M., Kim, S., Yu, J. - N., Kim, B. - J., Sakai, H., Lee, Y. J., and Suk, H. Y., Development of polymorphic microsatellite loci in Odontobutis obscura using Illumina paired-end sequencing and the test of cross-species amplification, vol. 15, p. -, 2016.

Odontobutis obscura is a bottom-dwelling freshwater fish native to East Asia. Its range encompasses southwest China, western Japan, and Geoje Island in South Korea. Despite its widespread range in China and Japan, only a small and spatially isolated population is found in South Korea. We developed a total of 23 novel and polymorphic microsatellite loci of O. obscura using Illumina paired-end shotgun sequencing and characterized them using 80 Japanese and Korean samples.

Genetic diversity of Lippia sidoides Cham. and L. gracilis Schauer germplasm

C. P. Santos, Pinheiro, J. B., Zucchi, M. I., Bajay, M. M., Campos, J. B., Arrigoni-Blank, M. F., Pinto, J. A. O., Blank, A. F., Santos, C. P., Pinheiro, J. B., Zucchi, M. I., Bajay, M. M., Campos, J. B., Arrigoni-Blank, M. F., Pinto, J. A. O., and Blank, A. F., Genetic diversity of Lippia sidoides Cham. and L. gracilis Schauer germplasm, vol. 15, p. -, 2016.

The conservation of plants in germplasm banks ensures the characterization and availability of these resources for future generations. The present study used DNA markers to obtain genetic information about germplasm collections of Lippia sidoides and L. gracilis, which are maintained in an Active Germplasm Bank (AGB). Genetic variability of samples in the AGB was assessed using 12 combinations of amplified fragment length polymorphism (AFLP) primers (EcoRI/MseI). Twenty simple sequence repeat primers designed for L.

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