Microsatellite

Development and characterization of 60 microsatellite markers in the abalone Haliotis diversicolor

Y. Wang, Wang, F., Shi, Y. H., Gu, Z. F., and Wang, A. M., Development and characterization of 60 microsatellite markers in the abalone Haliotis diversicolor, vol. 10, pp. 860-866, 2011.

The abalone, Haliotis diversicolor, is one of the most important mariculture species in southern China. We developed 60 new polymorphic microsatellite markers for H. diversicolor and characterized them in 30 individuals from a cultured population in Sanya, China. All 60 markers were found to be polymorphic. The number of alleles ranged from two to nine per locus, with an average of 4.12/locus. The expected and observed heterozygosities ranged from 0.10 to 0.88 and from 0.07 to 0.87, respectively. Forty-four loci were in Hardy-Weinberg equilibrium.

Polymorphisms of three new microsatellite sites of the dystrophin gene

R. F. Sun, Zhu, Y. S., Feng, J. L., Tian, Z., Kuang, W. J., Liu, Y., Zhang, H. B., and Li, S. B., Polymorphisms of three new microsatellite sites of the dystrophin gene, vol. 10, pp. 744-751, 2011.

To look for novel microsatellites in the dystrophin gene for the diagnosis of Duchenne muscular dystrophy, candidate microsatellite sites in the dystrophin gene were analyzed with the SSRHunter software and were also genotyped. Among the 15 candidate microsatellite sites, three novel microsatellite sites in the 60th, 30th, and 2nd intron were found to have a high degree of polymorphism. We submitted these three new loci to the European Molecular Biology Laboratory, under accession Nos.

Isolation and characterization of microsatellite loci in the fish Coilia mystus (Clupeiformes: Engraulidae) using PCR-based isolation of microsatellite arrays

J. - Q. Yang, Zhou, X. - D., Liu, D., Liu, Z. - Z., and Tang, W. - Q., Isolation and characterization of microsatellite loci in the fish Coilia mystus (Clupeiformes: Engraulidae) using PCR-based isolation of microsatellite arrays, vol. 10. pp. 1514-1517, 2011.

Coilia mystus is the most important harvested fish species in China; it inhabits quite different water environments during the different life history stages. Populations of C. mystus have dropped sharply due to overharvesting and water pollution. We developed eight microsatellite loci in C. mystus for conservation genetics studies. These new markers were tested in 20 individuals from the Min River in ChangLe. The number of alleles ranged from 3 to 8, the expected heterozygosity from 0.621 to 0.853 and the observed heterozygosity from 0.473-0.800.

Development and characterization of microsatellite markers for the lizardfish known as the Bombay duck, Harpadon nehereus (Synodontidae)

T. J. Xu, Sun, D. Q., Li, H. Y., and Wang, R. X., Development and characterization of microsatellite markers for the lizardfish known as the Bombay duck, Harpadon nehereus (Synodontidae), vol. 10, pp. 1701-1706, 2011.

The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety positive clones from the (CA)15-enriched genomic library were sequenced; 62 sequences contained sufficient repeat motifs (di-, tri- and tetra-nucleotide).

Isolation and characterization of tetranucleotide repeat polymorphic microsatellite loci in Larus saundersi (Aves, Laridae)

H. - X. Jiang, Ren, S. L., Hou, Y. - Q., Yang, M., and Wu, X. - B., Isolation and characterization of tetranucleotide repeat polymorphic microsatellite loci in Larus saundersi (Aves, Laridae), vol. 10. pp. 2034-2037, 2011.

Nine polymorphic microsatellite loci were isolated, using tetranucleotide repeat oligonucleotide probes from an enriched DNA library of the globally “vulnerable” Saunders’s gull (Larus saundersi), collected from the Yancheng coastal wetland, one of the three remaining breeding sites in China. Six breast muscle tissues and 16 blood samples from 22 gulls and eight eggshell membrane tissues were collected for this analysis. The number of alleles per locus ranged from 4 to 15, with a mean of 8.9.

Isolation and characterization of 19 polymorphic microsatellite loci from the topmouth gudgeon, Pseudorasbora parva

C. Zeng, Gul, Y., Yang, K., Cui, L., Wang, W. - M., and Gao, Z. - X., Isolation and characterization of 19 polymorphic microsatellite loci from the topmouth gudgeon, Pseudorasbora parva, vol. 10. pp. 1696-1700, 2011.

The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ezhou, Hubei province of China. The loci had 5 to 11 alleles, with a mean of 7.7 at each locus; 11 loci conformed to Hardy-Weinberg equilibrium.

Microsatellites behaving badly: empirical evaluation of genotyping errors and subsequent impacts on population studies

A. C. Kelly, Mateus-Pinilla, N. E., Douglas, M., Douglas, M., Shelton, P., and Novakofski, J., Microsatellites behaving badly: empirical evaluation of genotyping errors and subsequent impacts on population studies, vol. 10, pp. 2534-2553, 2011.

Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for population management and conservation programs.

Microsatellite analysis as a tool for discriminating an interfamily hybrid between olive flounder and starry flounder

J. - H. Kang, Kim, Y. - K., Park, J. - Y., An, C. - M., Nam, M. - M., Byun, S. G., Lee, B. I., Lee, J. H., and Choi, T. - J., Microsatellite analysis as a tool for discriminating an interfamily hybrid between olive flounder and starry flounder, vol. 10, pp. 2786-2794, 2011.

An interspecific artificial hybrid was produced between two economically important aquaculture flatfish: olive flounder (Paralichthys olivaceus) and starry flounder (P. stellatus). This hybrid displays the rapid growth characteristic of the former and tolerance to low temperatures and low salinity of the latter, but the genetics of inheritance in this hybrid have not been elucidated. Polymorphic microsatellite markers developed for P. olivaceus and P.

Polymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species

S. Q. Xuan, Zheng, J. Y., Wang, H., Gao, P., Zhou, S. B., Liu, K., and Zhu, G. - P., Polymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species, vol. 10, pp. 3141-3145, 2011.

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 microsatellite loci from 21 L. radiata individuals of a natural population from Yellow Mountain in Anhui Province, China. The number of alleles ranged from two to nine.

Genetic diversity analysis of an indigenous Chinese buffalo breed and hybrids based on microsatellite data

W. C. Yang, Tang, K. Q., Mei, J., Zeng, W. B., and Yang, L. G., Genetic diversity analysis of an indigenous Chinese buffalo breed and hybrids based on microsatellite data, vol. 10, pp. 3421-3426, 2011.

Chinese native buffaloes have faced the threat of extinction, along with an increase in crossbreeding with domesticated river buffaloes; consequently, conservation of local buffalo genetic resources has become a priority. A Chinese native breed, Jianghan, is often crossed intentionally and unintentionally with imported breeds from India and Pakistan, Murrah, and Nili-Ravi. A total of 128 buffaloes of the breeds Jianghan, Murrah, and Nili-Ravi and their presumed hybrid offspring were genotyped for 10 microsatellite markers.

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