Microsatellite

Development and characterization of microsatellite loci in Megalonibea fusca

Z. B. Li, Li, Q. H., Ning, Y. F., Shangguan, J. B., Dai, G., Chen, L. N., Cao, Y. Y., and Chen, X. J., “Development and characterization of microsatellite loci in Megalonibea fusca”, vol. 14, pp. 4633-4636, 2015.

Megalonibea fusca is a commercially important large edible fish. In this study, the first set of 10 polymorphic microsatellite loci for M. fusca was developed and characterized. The number of alleles per locus ranged from two to five, with the observed and expected heterozygosities ranging from 0.0667 to 0.7667, and from 0.0644 to 0.5828, respectively. Most of the loci were in Hardy-Weinberg equilibrium (P > 0.05), except for two loci (Mf25 and Mf30) after a Bonferroni’s correction (P

Screening the first set of polymorphic microsatellite loci in Lunella coronata granulata (Turbinidae)

Z. B. Li, Li, Q. H., Shangguan, J. B., Ning, Y. F., and Dai, G., “Screening the first set of polymorphic microsatellite loci in Lunella coronata granulata (Turbinidae)”, vol. 14. pp. 6319-6322, 2015.

Lunella coronata granulata, from the family Turbini­dae, is an economically important species. The first set of 10 poly­morphic microsatellite loci was screened from L. coronata granulata, and 30 individuals were used to analyze the degree of polymorphism in these loci. The level of observed and expected heterozygosity was 0.0667-0.7333 and 0.0644-0.6628, respectively. The polymorphism information content varied from 0.305 to 0.559.

Novel polymorphic microsatellite markers in Odontobutis potamophila

Y. B. Shen, Li, D., Li, J. L., Wang, R. Q., and Xuan, Y. F., “Novel polymorphic microsatellite markers in Odontobutis potamophila”, vol. 14, pp. 6265-6269, 2015.

We characterized 16 novel polymorphic loci isolated from a partial genomic DNA library of Odontobutis potamophila enriched for CA repeats. We tested the variability of these microsatellites on 51 unrelated individuals collected in China. All loci were polymorphic. The average allele number was 14.6 per locus, ranging from 2 to 27. The observed heterozygosity ranged from 0.35 to 0.90, with an average of 0.70, whereas the average expected heterozygosity was 0.76. Twelve of the 16 microsatellites conformed to Hardy-Weinberg equilibrium and were inherited independently.

Isolation and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria scabra

Z. B. Li, Dai, G., Shangguan, J. B., Ning, Y. F., Li, Y. Y., Chen, R. B., Huang, Y. S., and Yuan, Y., “Isolation and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria scabra”, vol. 14, pp. 6529-6532, 2015.

The sea cucumber Holothuria scabra is an endangered species. In this study, nine new polymorphic microsatellite loci were developed and tested in 30 individuals. The number of alleles ranged from 2 to 5, and the observed and expected heterozygosities ranged from 0.1200 to 0.7391 and from 0.2408 to 0.5983, respectively. No loci significantly deviated from the Hardy-Weinberg equilibrium af­ter a Bonferroni correction, and no significant linkage disequilibrium was found between pairs of loci.

Development and cross-species transferability of unigene-derived microsatellite markers in an edible oil woody plant, Camellia oleifera (Theaceae)

B. G. Jia, Lin, Q., Feng, Y. Z., Hu, X. Y., Tan, X. F., Shao, F. G., and Zhang, L., “Development and cross-species transferability of unigene-derived microsatellite markers in an edible oil woody plant, Camellia oleifera (Theaceae)”, vol. 14, pp. 6906-6916, 2015.

Camellia oleifera is an important edible oil woody plant in China. Lack of useful molecular markers hinders current genetic research on this tree species. Transcriptome sequencing of developing C. oleifera seeds generated 69,798 unigenes. A total of 6949 putative microsatellites were discovered among 6042 SSR-containing unigenes. Then, 150 simple sequence repeats (SSRs) were evaluated in 20 varieties of C. oleifera.

Investigation of Sclerotinia sclerotiorum strains variability in Brazil

M. J. Abreu and Souza, E. A., “Investigation of Sclerotinia sclerotiorum strains variability in Brazil”, vol. 14, pp. 6879-6896, 2015.

White mold is a common bean disease caused by the fungus Sclerotinia sclerotiorum, resulting in economic losses in Brazil and worldwide. Lack of knowledge about the population structure of the pathogen makes it difficult to control the disease. The aim of this study was to characterize strains of S. sclerotiorum obtained from ex­perimental and commercial common bean fields in Brazil. We analyzed 50 strains of S. sclerotiorum collected at several locations in the state of Minas Gerais.

Genetic differentiation between natural and hatchery populations of Manila clam (Ruditapes philippinarum) based on microsatellite markers

K. Xing, Gao, M. L., and Li, H. J., “Genetic differentiation between natural and hatchery populations of Manila clam (Ruditapes philippinarum) based on microsatellite markers”, vol. 13, pp. 237-245, 2014.

Manila clam (Ruditapes philippinarum) is one of the major aquaculture species around the world and supports an important segment of the aquaculture industry in China. In this study, we used ten microsatellite markers to detect genetic diversity within six R. philippinarum populations and genetic differentiation between them. A total of 109 alleles were detected across all loci.

Movement patterns and genetic diversity of wild and reintroduced common dormice, Muscardinus avellanarius

D. M. Naim, Telfer, S., Tatman, S., Bird, S., Kemp, S. J., and Watts, P. C., “Movement patterns and genetic diversity of wild and reintroduced common dormice, Muscardinus avellanarius”, vol. 13, pp. 167-181, 2014.

Movement is an important life history trait that can have an impact on local adaptation, and other evolutionary phenomena. We used a combination of nestbox survey data and genetic techniques (genotyping at 10 microsatellite loci) to quantify patterns of movement in common dormice Muscardinus avellanarius at two distinct sites in the UK: 1) Bontuchel (a natural population) and 2) Wych (captive-bred individuals that were reintroduced to this site), over three consecutive years (2006-2008).

Isolation and characterization of polymorphic microsatellite loci of the Chinese muntjac (Muntiacus reevesi)

H. Wang, Luo, X., Shi, W. B., and Zhang, B. W., “Isolation and characterization of polymorphic microsatellite loci of the Chinese muntjac (Muntiacus reevesi)”, vol. 13, pp. 1905-1908, 2014.

Eight polymorphic microsatellite markers for Muntiacus reevesi were identified and characterized in this study. The number of alleles per locus ranged from 3 to 10 across 24-48 samples. The loci showed expected and observed heterozygosities of 0.577-0.876 and 0.387-0.933, respectively, with an average polymorphic information content value of 0.682. These markers should be a useful tool for further population and conservation genetic studies of Muntiacus reevesi.

A multi-farm assessment of Greek black pig genetic diversity using microsatellite molecular markers

S. Michailidou, Kalivas, A., Ganopoulos, I., Stea, E., Michailidis, G., Tsaftaris, A., and Argiriou, A., “A multi-farm assessment of Greek black pig genetic diversity using microsatellite molecular markers”, vol. 13, pp. 2752-2765, 2014.

Local breeds are important for the maintenance of genetic diversity and future food security. Nowadays, the worldwide distribution of pigs is dominated by a few breeds, tending towards a severe loss of pig biodiversity. Thus, it is critical to maintain distinct populations of pig breeds. The Greek black pig, a breed raised locally and known for the high quality of its meat for cured products, is the only traditional indigenous pig breed reared in Greece.

Pages

Subscribe to Microsatellite