Microsatellite markers

Patterns of genetic diversity and population structure of the threatened Houbara and Macqueen’s bustards as revealed by microsatellite markers

A. Korrida, Jadallah, S., Chbel, F., Amin-Alami, A., Ahra, M., and Aggrey, S., Patterns of genetic diversity and population structure of the threatened Houbara and Macqueen’s bustards as revealed by microsatellite markers, vol. 11, pp. 3207-3221, 2012.

The Houbara bustard (Chlamydotis undulata) is a threatened avian species that is rapidly declining throughout its range, especially in North Africa, Asia and the Canary Islands. We examined the population structure and genetic variation for the three Houbara subspecies C. undulata undulata, C. u. fuertaventurae and C. u. macqueenii. A total of 266 birds from 10 populations were genotyped using seven polymorphic microsatellite markers. The analysis of microsatellite loci generated 1821 genotypes and 55 different alleles.

Genetic variability in Blanco Orejinegro breed cattle populations in Colombia

R. Martínez, Llinás, A. P., and M.-Rocha, J. F., Genetic variability in Blanco Orejinegro breed cattle populations in Colombia, vol. 12, pp. 1083-1094, 2013.

We analyzed population structure and genetic diversity in Blanco Orejinegro Creole cattle with 12 microsatellite markers, genotyping 138 individuals belonging to 7 commercial and 3 conservation herds. These markers showed a high level of polymorphism; 171 alleles were identified. The mean number of alleles per locus was 5.63 (3.82-6.58). The total number of alleles per marker was 14.2 and ranged from 16 (TGLA126) to 22 (TGLA227).

Genetic variability in Melipona scutellaris from Recôncavo, Bahia, Brazil

J. L. Viana, Francisco, A. K., Carvalho, C. A. L., and Waldschmidt, A. M., Genetic variability in Melipona scutellaris from Recôncavo, Bahia, Brazil, vol. 12, pp. 3444-3454, 2013.

Bees play a key role in pollination and thereby help maintain plant diversity. The stingless bee Melipona scutellaris is an important pollinator in northeastern Brazil because it is endemic to this region. Both deforestation and timber harvesting have reduced the nesting sites for this species, thus reducing its population and range. Genetic studies may help reverse this process by providing important tools for their proper management with a view to conservation of this species. Microsatellite markers have proven to be ideal for mapping genes and population genetic studies.

Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest

M. Novello, Rodrigues, J. F., Pinheiro, F., Oliveira, G. C. X., Veasey, E. A., and Koehler, S., Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest, vol. 12, pp. 3274-3278, 2013.

Microsatellite markers were developed for the endangered Brazilian orchid species Cattleya coccinea to describe its genetic diversity and structure and to support conservation studies. Nine microsatellite loci were isolated and characterized using an enriched genomic library. All loci are polymorphic at least in the 2 populations sampled, except for loci Cac05 and Cac09 for the Petrópolis population. The mean number of alleles per locus was 8.8 between populations.

Characterization and cross-species amplification of microsatellite markers in African Silverbill (Lonchura cantans)

N. R. Parine, Kumar, D., Pathan, A. A. K., Elrobh, M. S., Khan, W., and Alanazi, M., Characterization and cross-species amplification of microsatellite markers in African Silverbill (Lonchura cantans), vol. 12, pp. 5634-5639, 2013.

We tested the cross-amplification of eight microsatellites developed for Bengalese finch in African Silverbill (Lonchura cantans). In order to develop resources for conservation genetic studies in the species L. cantans, we tested the amplification success and polymorphism in eight previously developed microsatellite loci, in L. cantans. All eight microsatellite markers were successfully amplified, of which all were polymorphic, with 3 to 9 alleles and an expected heterozygosity (HE) ranging from 0.606 to 0.718.

Genetic diversity and differentiation of exotic and American commercial cattle breeds raised in Brazil

B. S. A. F. Brasil, Coelho, E. G. A., Drummond, M. G., and Oliveira, D. A. A., Genetic diversity and differentiation of exotic and American commercial cattle breeds raised in Brazil, vol. 12, pp. 5516-5526, 2013.

The Brazilian cattle population is mainly composed of breeds of zebuine origin and their American derivatives. Comprehensive knowledge about the genetic diversity of these populations is fundamental for animal breeding programs and the conservation of genetic resources. This study aimed to assess the phylogenetic relationships, levels of genetic diversity, and patterns of taurine/zebuine admixture among 9 commercial cattle breeds raised in Brazil.

Development and characterization of polymorphic microsatellite markers for Chinese raccoon dog (Nyctereutes procyonoides procyonoides)

S. Q. Yan, Li, Y. M., Bai, C. Y., Ding, X. M., Li, W. J., Hou, J. N., Zhao, Z. H., and Sun, J. H., Development and characterization of polymorphic microsatellite markers for Chinese raccoon dog (Nyctereutes procyonoides procyonoides), vol. 12, pp. 6351-6355, 2013.

Chinese raccoon dog (Nyctereutes procyonoides procyonoides) is one of the most important fur-bearing animal species. Information about the genetic background of farmed Chinese raccoon dogs is limited. In this study, 17 polymorphic microsatellite markers were isolated and identified from an (AC)n-microsatellite-enriched library of Chinese raccoon dogs. The number of alleles per locus ranged from 2 to 8 based on 48 individuals tested.

Transferability of microsatellite markers among economically and ecologically important galliform birds

N. Bech, Novoa, C., Allienne, J. F., and Boissier, J., Transferability of microsatellite markers among economically and ecologically important galliform birds, vol. 9, pp. 1121-1129, 2010.

We used the partially sequenced genomes of the turkey and chicken to find a large number of microsatellite markers. We then characterized 10 polymorphic microsatellite markers developed by cross-species amplification from economically and ecologically important birds to various European sub-species of the grey partridge. Even though we used cross-species amplification, a high degree of polymorphism was conserved in all microsatellite markers.

Genetic characterization of papaya plants (Carica papaya L.) derived from the first backcross generation

H. C. C. Ramos, Pereira, M. G., Silva, F. F., Gonçalves, L. S. A., Pinto, F. O., Filho, G. Ade Souza, and Pereira, T. S. N., Genetic characterization of papaya plants (Carica papaya L.) derived from the first backcross generation, vol. 10, pp. 393-403, 2011.

The limited number of papaya varieties available reflects the narrow genetic base of this species. The use of backcrossing as a breeding strategy can promote increases in variability, besides allowing targeted improvements. Procedures that combine the use of molecular markers and backcrossing permit a reduction of the time required for introgression of genes of interest and appropriate recovery of the recurrent genome.

Isolation and characterization of eight polymorphic microsatellite loci for the coconut pest, Brontispa longissima (Coleoptera: Hispidae)

C. L. Ma, Wu, H. L., Hu, H. Y., Wu, X., Ma, G. C., Fu, Y. G., and Peng, Z. Q., Isolation and characterization of eight polymorphic microsatellite loci for the coconut pest, Brontispa longissima (Coleoptera: Hispidae), vol. 10. pp. 429-432, 2011.

Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia; it was first discovered on Hainan Island in June 2002. Despite the economic risk associated with this pest, genetic aspects of the invasion process have remained relatively unexplored. Using microsatellite markers, we investigated the population structure, genetic variability and pattern of invasion in various geographic populations. The methodology was based on a modified biotin-capture method. Eight polymorphic microsatellite loci were isolated and characterized for the pest.

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