Microsatellite marker

Isolation and characterization of novel polymorphic microsatellite markers for Epinephelus akaara

Z. Z. Xie, Zheng, L. Y., Tang, L., Tang, Z. J., Li, S. S., Zhang, Y., and Lin, H. R., Isolation and characterization of novel polymorphic microsatellite markers for Epinephelus akaara, vol. 14, pp. 13663-13666, 2015.

In the present study, ten novel microsatellite markers were developed from an enriched-(CA)13 genomic library of Epinephelus akaara. The mean number of alleles per locus was 21.6, with a range of 12 to 33. Observed heterozygosity ranged from 0.767 to 0.967, and expected heterozygosity ranged from 0.831 to 0.975, with mean values of 0.877 and 0.923, respectively. Among the ten loci, three loci deviated from Hardy-Weinberg equilibrium after sequential Bonferroni’s correction.

Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin

Y. - X. Liu, Ma, C. - Y., Ma, H. - Y., Feng, C. - L., Li, S. - J., and Ma, L. - B., Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin, vol. 14, pp. 15320-15324, 2015.

Nine microsatellite DNA markers were developed and characterized for Siganus oramin by the 5'-anchored polymerase chain reaction technique. A total of 42 alleles were identified in 30 individuals, and the number of alleles per locus ranged from 3 to 7, with an average of 4.7. The observed and expected heterozygosity per locus ranged from 0.5333 to 1.0000 and from 0.5254 to 0.8474, respectively, with an average of 0.7422 and 0.6906, respectively.

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