Microsatellite loci

Microsatellite markers for Amazon pellona Pellona castelnaeana (Clupeiformes: Pristigasteridae)

A. M. Ximenes, Hernández-Ruz, E. J., Machado, V. N., Rodrigues, L. R. R., Ritter, G. C. S., and Farias, I. P., Microsatellite markers for Amazon pellona Pellona castelnaeana (Clupeiformes: Pristigasteridae), vol. 14. pp. 5123-5126, 2015.

The Amazon pellona is one of the few species of Pristigasteridae with recognized commercial value in the Amazon. We isolated 24 and characterized 8 microsatellite loci for this species. The number of alleles ranges from 2-8 per locus. Observed heterozygosities ranged from 0.052-0.823, while expected heterozygosities from 0.052-0.836. These 8 microsatellites are potentially valuable tools for characterizing the levels and distribution of genetic diversity, population structure, and gene flow.

Isolation and characterization of polymorphic microsatellite loci from aerial yam (Dioscorea bulbifera L.)

Q. Q. Yan, Li, Y., Sun, X. Q., Guo, J. L., Hang, Y. Y., and Li, M. M., Isolation and characterization of polymorphic microsatellite loci from aerial yam (Dioscorea bulbifera L.), vol. 13, pp. 1514-1517, 2014.

Dioscorea bulbifera L. is widely distributed in pantropical regions along the equator. The taxonomic treatment of this species is ambiguous due to its extreme polymorphic morphological characters. In order to provide tools to facilitate the study of genetic diversity, population structure, patterns of gene flow, and the mating system of this species, and to assess intraspecific variability and relationships in D. bulbifera, 14 novel polymorphic microsatellite loci were developed using the dual-suppression PCR technique.

Polymorphic microsatellite loci for the crimson snapper (Lutjanus erythropterus)

L. Liu, Lin, L., Li, C. H., Xu, S. N., Liu, Y., and Zhou, Y. B., Polymorphic microsatellite loci for the crimson snapper (Lutjanus erythropterus), vol. 13, pp. 5250-5253, 2014.

We isolated and characterized 22 polymorphic microsatellite loci in Lutjanus erythropterus using a (GT)13-enriched genomic library. We found between 2 and 8 alleles per locus, with a mean of 4.85. The observed and expected heterozygosities ranged from 0.065 to 0.867 and from 0.085 to 0.832, respectively, with means of 0.461 and 0.529, respectively. Allele frequencies in three loci were found to deviate from Hardy-Weinberg equilibrium. Evidence for null alleles was found for three loci. These markers will be useful for distinguishing released captive-bred L.

Genetic characterization of hatchery populations of Korean spotted sea bass (Lateolabrax maculatus) using multiplex polymerase chain reaction assays

H. S. An, Kim, H. Y., Kim, J. B., Chang, D. S., Park, K. D., Lee, J. W., Myeong, J. I., and An, C. M., Genetic characterization of hatchery populations of Korean spotted sea bass (Lateolabrax maculatus) using multiplex polymerase chain reaction assays, vol. 13, pp. 6701-6715, 2014.

The spotted sea bass, Lateolabrax maculatus, is an important commercial and recreational fishery resource in Korea. Aquacultural production of this species has increased because of recent resource declines, growing consumption, and ongoing government-operated stock release programs. Therefore, the genetic characterization of hatchery populations is necessary to maintain the genetic diversity of this species and to develop more effective aquaculture practices.

Characterization of novel microsatellite markers derived from Korean rose bitterling (Rhodeus uyekii) genomic library

W. J. Kim, Shin, E. H., Kong, H. J., Kim, H. S., Kim, B. S., Nam, B. H., Kim, Y. O., Kim, C. H., Jung, H., and An, C. M., Characterization of novel microsatellite markers derived from Korean rose bitterling (Rhodeus uyekii) genomic library, vol. 13, pp. 8147-8152, 2014.

Korean rose bitterling (Rhodeus uyekii) is a freshwater fish endemic to Korea. Natural populations of this species have experienced severe declines as a result of habitat fragmentation and water pollution. To conserve and restore R. uyekii, the genetic diversity of this species needs to be assessed at the population level. Eighteen novel polymorphic microsatellite loci for R. uyekii were developed using an enriched partial genomic library. Polymorphisms at these loci were studied in 150 individuals collected from three populations.

Genetic differences between the wild and hatchery-produced populations of Korean short barbeled grunter (Hapalogenys nitens) determined with microsatellite markers

H. S. An, Kang, H. W., Han, H. S., Park, J. Y., Hong, C. G., Park, J., Myeong, J. I., and An, C. M., Genetic differences between the wild and hatchery-produced populations of Korean short barbeled grunter (Hapalogenys nitens) determined with microsatellite markers, vol. 13, pp. 8901-8912, 2014.

Short barbeled grunter, Hapalogenys nitens, is an economically important fishery resource. In Korea, this fish is in the early stage of domestication, and it has been regarded as the candidate marine fish species for prospective aquaculture diversification. This study presents a preliminary investigation of the future viability of sustainable fry production from short barbeled grunter.

Comparison of genetic diversity between wild-caught broodstock and hatchery-produced offspring populations of the vulnerable Korean kelp grouper (Epinephelus bruneus) by microsatellites

H. S. An, Yang, S. G., Moon, T. S., Park, J. Y., Hong, C. G., Hwang, H. K., Myeong, J. I., and An, C. M., Comparison of genetic diversity between wild-caught broodstock and hatchery-produced offspring populations of the vulnerable Korean kelp grouper (Epinephelus bruneus) by microsatellites, vol. 13, pp. 9675-9686, 2014.

The kelp grouper Epinephelus bruneus (Perciformes: Haemulidae), is one of the most economically important fishery resources in Korea. This fish is regarded as a target for prospective aquaculture diversification; therefore, maintenance of stock quality is important. To investigate the effects of current artificial reproduction in a hatchery facility, genetic variation in wild-caught broodstock and hatchery-produced offspring of kelp grouper was analyzed using eight polymorphic nuclear microsatellite DNA loci; 77 alleles were identified.

Isolation and characterization of polymorphic microsatellite loci from Zelkova schneideriana Hand.-Mazz.

H. L. Liu, Zhang, R. Q., Geng, M. L., Zhu, J. Y., and Ma, J. L., Isolation and characterization of polymorphic microsatellite loci from Zelkova schneideriana Hand.-Mazz., vol. 13, pp. 10062-10066, 2014.

Zelkova schneideriana is a highly valued hardwood species. An improved technique for isolating codominant compound microsatellite markers was used to develop simple sequence repeat markers for Z. schneideriana. A total of 12 microsatellite loci were identified. Overall, the number of alleles per locus ranged from 8-19, with an average of 11.75. Observed heterozygosity and expected heterozygosity values ranged from 0.109-0.709 and 0.832-0.929, respectively. Polymorphic information content is from 0.803-0.915, with an average of 0.854.

Novel polymorphic microsatellite markers isolated from the pen shell Atrina pectinata (Mollusca: Bivalvia: Pinnidae)

B. Wu, Bai, L. J., Yang, A. G., Zhou, L. Q., and Liu, Z. H., Novel polymorphic microsatellite markers isolated from the pen shell Atrina pectinata (Mollusca: Bivalvia: Pinnidae), vol. 13, pp. 10643-10647, 2014.

In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism information content (PIC) varied from 0.1730 to 0.8954.

Development of microsatellite markers for the small yellow croaker Larimichthys polyactis (Sciaenidae) by cross-species amplification

D. Q. Sun, Li, H. Y., Xu, T. J., and Wang, R. X., Development of microsatellite markers for the small yellow croaker Larimichthys polyactis (Sciaenidae) by cross-species amplification, vol. 11, pp. 1469-1474, 2012.

The small yellow croaker (Larimichthys polyactis) is a highly valued fish for human consumption found in the Western Pacific that was considered endangered until recently because of overfishing. We selected microsatellite markers for this species from markers developed for Miichthys miiuy, also of the family Sciaenidae. Among 43 markers polymorphic for M. miiuy, 11 were found to be polymorphic for L. polyactis. Characterization of these 11 loci was made based on 30 L.

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