Microsatellite DNA

Development of polymorphic microsatellite loci for a new fish species, Chinese sillago (Sillago sinica)

N. Song, Wang, L. Y., Yin, L. N., Han, Z. Q., Gao, T. X., Song, N., Wang, L. Y., Yin, L. N., Han, Z. Q., Gao, T. X., Song, N., Wang, L. Y., Yin, L. N., Han, Z. Q., and Gao, T. X., Development of polymorphic microsatellite loci for a new fish species, Chinese sillago (Sillago sinica), vol. 15, p. -, 2016.

Sillago sinica is a newly identified species belonging to Sillaginidae, Perciforms, and was found along the coast of China in 2011. In the present study, 81 microsatellite loci were isolated from an enriched genomic library, and 24 positive clones containing microsatellite repeats had adequate flanking sequences for the development of PCR primers. Sixteen of these primers were monomorphic or would not amplify. Eight were polymorphic in an examined population with the number of alleles per locus ranging from 2 to 14.

Development of novel DNA markers for genetic analysis of grey hamsters by cross-species amplification of microsatellites

C. Wang, Zhang, S. J., Du, X. Y., Xu, Y. M., Huo, X. Y., Liao, L. F., and Chen, Z. W., Development of novel DNA markers for genetic analysis of grey hamsters by cross-species amplification of microsatellites, vol. 14, pp. 14339-14347, 2015.

The grey hamster has been used in biomedical research for decades. However, effective molecular methods for evaluating the genetic structure of this species are lacking, which hinders its wider usage. In this study, we employed cross-amplification of microsatellite loci of species within the same genus by polymerase chain reaction. Loci screened included 107 from the Mongolian gerbil (MG) and 60 from the Chinese hamster (CH); of these, 15 polymorphic loci were identified for the grey hamster.

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