Microsatellite analysis

Genetic diversity among melon accessions (Cucumis melo) from Turkey based on SSR markers

Y. A. Kaçar, Simsek, O., Solmaz, I., Sari, N., and Mendi, Y. Y., “Genetic diversity among melon accessions (Cucumis melo) from Turkey based on SSR markers”, vol. 11, pp. 4622-4631, 2012.

Melon (Cucumis melo) is an important vegetable crop in Turkey, where it is grown in many regions; the most widely planted lines are local winter types belonging to the var. inodorous. We examined 81 melon genotypes collected from different provinces of Turkey, compared with 15 reference melon genotypes obtained from INRA/France, to determine genetic diversity among Turkish melons. Twenty polymorphic primers were used to generate the SSR markers. PCR amplification was performed and electrophoresis was conducted. SSR data were used to generate a binary matrix.

A combination of five short tandem repeats of chromosome 15 significantly improves the identification of Prader-Willi syndrome etiology in the Argentinean population

H. V. Aráoz, Torrado, M., Barreiro, C., and Chertkoff, L., “A combination of five short tandem repeats of chromosome 15 significantly improves the identification of Prader-Willi syndrome etiology in the Argentinean population”, vol. 5, pp. 390-398, 2006.

Prader-Willi syndrome (PWS) is a multisystemic disorder caused by the loss of expression of paternally transcribed genes in the PWS critical region of chromosome 15. Various molecular mechanisms are known to lead to PWS: deletion 15q11-q13 (75% of cases), maternal uniparental disomy (matUPD15) (23%) and imprinting defects (2%). FISH and microsatellite analysis are required to establish the molecular etiology, which is essential for appropriate genetic counseling and care management.

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