Microsatellite

Dynamic comparison of genetic diversity in a Small Tail Han sheep population using meta-analysis

G. X. E, Huang, Y. F., Zhao, Y. J., He, J. N., Liu, N., Zhong, T., Ma, Y. H., Qiu, X. Y., and Chen, L. P., Dynamic comparison of genetic diversity in a Small Tail Han sheep population using meta-analysis, vol. 14, pp. 14607-14614, 2015.

The aim of this research was to identify the dynamic diversity of Small Tail Han sheep in its main producing areas between different years, and provide a basis for a breeding and genetic resources conservation strategy. For this purpose, 15 microsatellites were genotyped for Small Tail Han Sheep sampled in 2014 from Heze, China, and a comparative analysis of these data with those from a previous study was undertaken using meta-analysis. The results reveal that inbreeding has caused a reduction in diversity of Small Tail Han Sheep from 2008 to 2014.

Efficient development of polymorphic microsatellite loci for Pteroceltis tatarinowii (Ulmaceae)

X. H. Li, Zhang, X. P., Liu, K., Liu, H. J., and Shao, J. W., Efficient development of polymorphic microsatellite loci for Pteroceltis tatarinowii (Ulmaceae), vol. 14, pp. 16444-16449, 2015.

Pteroceltis tatarinowii (Ulmaceae) is a scientifically and economically important temperate deciduous tree that is endemic to China. In the present study, 12 P. tatarinowii polymorphic microsatellite loci were developed using the tailed primer-M13-simple sequence repeats (TP-M13-SSR) biotin-capture method. The number of alleles per locus ranged from 2 to 10, with an average of 6.58. The observed and expected heterozygosity ranged from 0.208 to 0.958 and from 0.198 to 0.858, with average values of 0.703 and 0.710, respectively.

Genetic parameters and mapping quantitative trait loci associated with tibia traits in broilers

B. N. N. Ragognetti, Stafuzza, N. B., Chud, T. C. S., Grupioni, N. V., Cruz, V. A. R., Peixoto, J. O., Nones, K., Ledur, M. C., Munari, D. P., and Silva, T. B. R., Genetic parameters and mapping quantitative trait loci associated with tibia traits in broilers, vol. 14, pp. 17544-17554, 2015.

Selection among broilers for performance traits is resulting in locomotion problems and bone disorders, once skeletal structure is not strong enough to support body weight in broilers with high growth rates. In this study, genetic parameters were estimated for body weight at 42 days of age (BW42), and tibia traits (length, width, and weight) in a population of broiler chickens. Quantitative trait loci (QTL) were identified for tibia traits to expand our knowledge of the genetic architecture of the broiler population.

Microsatellite DNA markers and their correlation with growth traits in mandarin fish (Siniperca chuatsi)

L. F. Sun, Li, J., Liang, X. F., Yi, T. L., Fang, L., Sun, J., He, Y. H., Luo, X. N., Dou, Y. Q., and Yang, M., Microsatellite DNA markers and their correlation with growth traits in mandarin fish (Siniperca chuatsi), vol. 14, pp. 19128-19135, 2015.

The mandarin fish (Siniperca chuatsi) is a traditionally cultured freshwater fish with high commercial value in China. To facilitate marker-assisted selection in genetic improvement of this species, 120 microsatellite markers from the literature were characterized in the 25 largest and 25 smallest individuals. Eighteen polymorphic loci were then used to genotype 200 individuals, and the associations between their genotypes and growth traits were examined.

Isolation and characterization of microsatellite loci in the purpleback flying squid (Sthenoteuthis oualaniensi)

L. Lin, Li, P., Chen, Z. Z., Xiao, Y. Y., Xu, S. N., Liu, Y., and Li, C. H., Isolation and characterization of microsatellite loci in the purpleback flying squid (Sthenoteuthis oualaniensi), vol. 14. pp. 7630-7633, 2015.

The purpleback flying squid (Sthenoteuthis oualaniensis) is a pelagic squid with tremendous potential for commercial exploitation. We isolated and characterized 21 polymorphic microsatellite loci for S. oualaniensis using a (GT)13-enriched genomic library. The number of alleles per locus varied from 6 to 32. The observed and expected heterozygosities ranged from 0.188 to 0.890, and 0.537 to 0.968, respectively. No significant linkage disequilibrium was detected at these loci.

Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology

L. M. Li, Wei, L., Jiang, H. Y., Zhang, Y., Zhang, X. J., Yuan, L. H., and Chen, J. P., Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology, vol. 14, pp. 7910-7913, 2015.

Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more infor­mation on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the develop­ment of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of 24 individuals collected from a sturgeon farm. The results show that the mean number of ob­served alleles per locus is 6.6 (ranging from 2 to 17).

Development and characterization of microsatellite markers for molecular genetic diversity in Atrina pectinata

Y. F. Ning, Li, Z. B., Shangguan, J. B., Huang, Y. S., Jiang, P. D., Xu, A. L., and Yuan, Y., Development and characterization of microsatellite markers for molecular genetic diversity in Atrina pectinata, vol. 14, pp. 8290-8293, 2015.

The pen shell, Atrina pectinata, is an economically valuable species that is widely distributed along the coastal waters of temperate and tropical areas, mainly growing in the Indian and Pacific Oceans. Eight novel microsatellite loci from the genome of A. pectinata were developed using fast isolation by amplified fragment length polymorphism of sequence containing repeats. The loci were screened in 30 wild individuals.

Characterization of microsatellite markers and their correlations with growth traits in Mandarin fish (Siniperca chuatsi)

T. L. Yi, Fang, L., Liang, X. F., Sun, L. F., Li, J., Luo, X. N., Guo, W. J., Dou, Y. Q., and Sun, J., Characterization of microsatellite markers and their correlations with growth traits in Mandarin fish (Siniperca chuatsi), vol. 14, pp. 8926-8934, 2015.

Mandarin fish (Siniperca chuatsi) is a traditionally cultured freshwater fish with high commercial value in China. To facilitate marker-assisted selection for genetic improvement of this species, 100 microsatellite markers identified in previous studies were characterized in the 25 largest and 25 smallest individuals. Twenty polymorphic loci were used to genotype 200 individuals, and the associations between their genotypes and growth traits were examined.

Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi

C. F. Sun, Ye, X., Tian, Y. Y., and Dong, J. J., Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi, vol. 14, pp. 9343-9352, 2015.

In order to provide genetic information for the selective breeding of Siniperca chuatsi, 14 microsatellite DNA loci were used to evaluate the genetic diversity and structure of four farmed populations and one wild population in China. The four cultivated populations were Foshan (FS), Jiangmen (JM), Nanjing (NJ), and Hongze Lake (HZL), and the wild population was collected from the Hubei HuangGang section of the Yangtze River (HG).

Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus)

Z. B. Li, Huang, Y. S., Shangguan, J. B., Ning, Y. F., Yi, Y., and Dai, G., Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus), vol. 14, pp. 10224-10227, 2015.

Branchiostoma belcheri Gray is a second-class, nationally protected protochordate in China. We developed 10 novel polymorphic sites in B. belcheri, which were examined using a population of 30 wild individuals from Xiamen, China. The polymorphism information content ranged between 0.141 and 0.681, and the number of alleles varied from 2 to 5. The expected and observed heterozygosities varied between 0.1528 and 0.6920, and between 0.1429 and 0.5000, respectively. These novel microsatellite markers will facilitate the genetic analysis and protection of wild B.

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