Microsatellite

Genetic diversity in fragmented populations of Populus talassica inferred from microsatellites: implications for conservation

X. H. Zhu, Cheng, S. P., Liao, T., Kang, X. Y., Zhu, X. H., Cheng, S. P., Liao, T., and Kang, X. Y., “Genetic diversity in fragmented populations of Populus talassica inferred from microsatellites: implications for conservation”, vol. 15, p. -, 2016.

Populus talassica Kom. is an ecologically important species endemic to central Asia. In China, its main distribution is restricted to the Ili region in the Xinjiang Autonomous Region. An understanding of genetic diversity and population structure is crucial for the development of a feasible conservation strategy. Twenty-six high-level simple sequence repeat (SSR) markers were screened and used to genotype 220 individuals from three native populations. A high level of genetic diversity and low population differentiation were revealed.

Genetic diversity among air yam (Dioscorea bulbifera) varieties based on single sequence repeat markers

D. M. Silva, Siqueira, M. V. B. M., Carrasco, N. F., Mantello, C. C., Nascimento, W. F., Veasey, E. A., Silva, D. M., Siqueira, M. V. B. M., Carrasco, N. F., Mantello, C. C., Nascimento, W. F., and Veasey, E. A., “Genetic diversity among air yam (Dioscorea bulbifera) varieties based on single sequence repeat markers”, vol. 15, p. -, 2016.

Dioscorea is the largest genus in the Dioscoreaceae family, and includes a number of economically important species including the air yam, D. bulbifera L. This study aimed to develop new single sequence repeat primers and characterize the genetic diversity of local varieties that originated in several municipalities of Brazil. We developed an enriched genomic library for D. bulbifera resulting in seven primers, six of which were polymorphic, and added four polymorphic loci developed for other Dioscorea species.

High polymorphism at microsatellite loci in the Chinese donkey

R. F. Zhang, Xie, W. M., Zhang, T., Lei, C. Z., Zhang, R. F., Xie, W. M., Zhang, T., and Lei, C. Z., “High polymorphism at microsatellite loci in the Chinese donkey”, vol. 15, p. -, 2016.

To reveal the genetic diversity and phylogenetic relationships between Chinese donkey breeds, 415 individuals representing ten breeds were investigated using ten microsatellite markers. The observed number of alleles, mean effective number of alleles (NE), mean expected heterozygosity (HE), and polymorphic information content (PIC) of each breed and polymorphic locus were analyzed. The results showed that seven (HTG7, HTG10, AHT4, HTG6, HMS6, HMS3, and HMS7) of ten microsatellite loci were polymorphic.

Ancestry analysis of locally adapted Crespa goats from southernmost Brazil

D. D. Lopes, Fernández, G. P., Poli, M., Moreira, G. R. P., Gonçalves, G. L., Freitas, T. R. O., Lopes, D. D., Fernández, G. P., Poli, M., Moreira, G. R. P., Gonçalves, G. L., and Freitas, T. R. O., “Ancestry analysis of locally adapted Crespa goats from southernmost Brazil”, vol. 15, p. -, 2016.

Crespa goats are phenotypically similar to the Angora breed, and are traditionally reared in small, low-tech farms in southernmost Brazil. Whether they represent degenerated remnants of pure Angora goats or result from foreign breeds introduced during colonial times and recently mixed with commercial breeds is unknown.

Cross-species amplification and polymorphism of microsatellite loci in Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) in Brazilian cropping systems

N. A. Leite, Corrêa, A. S., Alves-Pereira, A., Campos, J. B., Zucchi, M. I., Omoto, C., Leite, N. A., Corrêa, A. S., Alves-Pereira, A., Campos, J. B., Zucchi, M. I., Omoto, C., Leite, N. A., Corrêa, A. S., Alves-Pereira, A., Campos, J. B., Zucchi, M. I., and Omoto, C., “Cross-species amplification and polymorphism of microsatellite loci in Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) in Brazilian cropping systems”, vol. 15, p. -, 2016.

The Old World bollworm Helicoverpa armigera (Hübner) was recently discovered in Brazil. This species is closely related to the New World bollworm H. zea (Boddie), and mating between these species has already been reported under laboratory conditions. Here, we tested the cross-species amplification of 20 microsatellite (SSR) loci in field populations of H. armigera and H. zea collected from Brazilian cropping systems.

Microsatellite markers reveal genetic divergence among wild and cultured populations of Chinese sucker Myxocyprinus asiaticus

W. W. Cheng, Wang, D. Q., Wang, C. Y., Du, H., Wei, Q. W., Cheng, W. W., Wang, D. Q., Wang, C. Y., Du, H., Wei, Q. W., Cheng, W. W., Wang, D. Q., Wang, C. Y., Du, H., and Wei, Q. W., “Microsatellite markers reveal genetic divergence among wild and cultured populations of Chinese sucker Myxocyprinus asiaticus”, vol. 15, p. -, 2016.

Studies of genetic diversity and genetic population structure are critical for the conservation and management of endangered species. The Chinese sucker Myxocyprinus asiaticus is a vulnerable monotypic species in China, which is at a risk of decline owing to fluctuations in effective population size and other demographic and environmental factors. We screened 11 microsatellite loci in 214 individuals to assess genetic differentiation in both wild and cultured populations.

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