Methyltransferase

Role of heme oxygenase-1 in demethylating effects on SKM-1 cells induced by decitabine

R. Gao, Ma, D., Wang, P., Sun, J., Wang, J. S., and Fang, Q., Role of heme oxygenase-1 in demethylating effects on SKM-1 cells induced by decitabine, vol. 14, pp. 17788-17798, 2015.

We evaluated the influence of heme oxygenase-1 (HO-1) gene inhibition in myelodysplastic syndrome (MDS) cell line SKM-1 on enhancement of the demethylating effects of decitabine on p15, and explored the possible mechanism. DNMT1 gene expression in SKM-1 cells was silenced by being transfected by a constructed siRNA with liposomes. The proliferation inhibition rates after drug treatment were detected by cell counting kit-8 assay. The apoptotic rates were detected by Annexin V/PI assay with flow cytometry.

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