Methylation-sensitive amplification polymorphism

Analysis of DNA methylation patterns and levels in maize hybrids and their parents

T. J. Liu, Sun, L. F., Shan, X. H., Wu, Y., Su, S. Z., Li, S. P., Liu, H. K., Han, J. Y., and Yuan, Y. P., “Analysis of DNA methylation patterns and levels in maize hybrids and their parents”, vol. 13, pp. 8458-8468, 2014.

Heterosis is the superior performance of heterozygous individuals and has been widely exploited in plant breeding, although the underlying regulatory mechanisms still remain largely elusive. To understand the molecular basis of heterosis in maize, in this study, roots and leaves at the seedling stage and embryos and endosperm tissues 15 days after fertilization of 2 elite hybrids and their parental lines were used to estimate the levels and patterns of cytosine methylation by the methylation-sensitive amplification polymorphism method.

Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism

T. Kanchanaketu, Sangduen, N., Toojinda, T., and Hongtrakul, V., “Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism”, vol. 11, pp. 944-955, 2012.

Genetic analysis of 56 samples of Jatropha curcas L. collected from Thailand and other countries was performed using the methylation-sensitive amplification polymorphism (MSAP) technique. Nine primer combinations were used to generate MSAP fingerprints. When the data were interpreted as amplified fragment length polymorphism (AFLP) markers, 471 markers were scored. All 56 samples were classified into three major groups: γ-irradiated, non-toxic and toxic accessions.

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