Method evaluation

Optimizing the efficiency of the touchdown technique for detecting inter-simple sequence repeat markers in corn (Zea mays)

E. C. Oliveira, Júnior, A. T. Amaral, Gonçalves, L. S. A., Pena, G. F., Júnior, S. P. Freitas, Ribeiro, R. M., and Pereira, M. G., Optimizing the efficiency of the touchdown technique for detecting inter-simple sequence repeat markers in corn (Zea mays), vol. 9, pp. 835-842, 2010.

We evaluated the efficiency of the touchdown method to determine the ideal PCR conditions for distinct inter-simple sequence repeat primers for processing DNA from common corn, popcorn, sweet corn, and a Tripsacum-maize hybrid. Genomic DNA was extracted from eight accessions of corn: two of the dent type, one Tripsacum-maize hybrid, one sweet corn, one flint-type corn, and three popcorn.

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