Metastasis

NDC80 promotes proliferation and metastasis of colon cancer cells

X. K. Xing, Wu, H. Y., Chen, H. L., Feng, H. G., Xing, X. K., Wu, H. Y., Chen, H. L., and Feng, H. G., NDC80 promotes proliferation and metastasis of colon cancer cells, vol. 15, p. -, 2016.

Chromosome instability is a common feature of tumor cells, and may be an important mechanism in tumor formation. Nuclear division cycle 80 (NDC80) is closely associated with the stability of chromosomes. Therefore, we investigated the relationship between NDC80 and development of colon cancer using a range of methods.

Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells

B. K. Li, Guo, K., Li, C. Y., Li, H. L., Zhao, P. P., Chen, K., and Liu, C. X., Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells, vol. 14, pp. 15769-15778, 2015.

This study investigated CapG gene expression in prostate cancer cell lines; in addition, we explored the effects of CapG suppression on DU145 cell growth, and the underlying mechanism with which CapG affects DU145 cell growth and invasiveness. The expression of CapG and 18 related genes in DU145 cells was analyzed by flow cytometry, quantitative polymerase chain reaction (qPCR), CCK8 assay, western blot, and the trans-well assay. DU145 cells were transfected with designed small interfering RNA (siRNA). CapG expression was quantified by qPCR and western blot.

Establishment of a neuroblastoma mouse model by subcutaneous xenograft transplantation and its use to study metastatic neuroblastoma

Q. Gao, Chen, C. F., Dong, Q., Hou, L., Chen, X., Zhi, Y. L., Li, X., Lu, H. T., and Zhang, H. Y., Establishment of a neuroblastoma mouse model by subcutaneous xenograft transplantation and its use to study metastatic neuroblastoma, vol. 14, pp. 16297-16307, 2015.

The aim of this study was to establish a metastatic human neuroblastoma (NB) mouse model by xenograft in order to study the metastatic mechanisms of NB. A human NB cell line was obtained from a 5-year-old patient and cultured in vitro. A suspension of these cells was subcutaneously inoculated into nude mice at the right flank next to the forelimb. The biological characteristics of the developed subcutaneous and metastatic tumors were analyzed by hematoxylin and eosin staining.

Clinical research on dendritic cell vaccines to prevent postoperative recurrence and metastasis of liver cancer

T. Y. Sun, Yan, W., Yang, C. M., Zhang, L. F., Tang, H. L., Chen, Y., Hu, H. X., and Wei, X., Clinical research on dendritic cell vaccines to prevent postoperative recurrence and metastasis of liver cancer, vol. 14, pp. 16222-16232, 2015.

We aimed to evaluate dendritic cell (DC) tumor vaccines for preventing liver cancer recurrence and metastasis. DCs were induced from mononuclear cells in the peripheral blood with recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) and recombinant human interleukin 4 (rhIL-4), followed by sensitization with lysis of autologous liver cancer cells. One hundred and sixty patients with hepatocellular carcinoma were randomly divided into two groups of 80. One group was treated postoperatively with six cycles of the DC tumor vaccine.

Expression levels of survivin, Bcl-2, and KAI1 proteins in cervical cancer and their correlation with metastasis

X. L. Zhou and Wang, M., Expression levels of survivin, Bcl-2, and KAI1 proteins in cervical cancer and their correlation with metastasis, vol. 14, pp. 17059-17067, 2015.

Cervical cancer is associated with abnormal expression of multiple genes. Survivin and Bcl-2 proteins are apoptosis inhibitors. The tumor suppressor gene CD82, which encodes the protein KAI1, is downregulated in cervical cancer, and is associated with differentiation degree. We investigated the expression levels of three proteins and their correlation with metastasis in cervical cancer by comparing them in different cervical lesions.

Decreased miR-134 expression and its tumor-suppressive function in human osteosarcoma

Y. Bao, Peng, L., Ma, J., Liu, K., and Li, W., Decreased miR-134 expression and its tumor-suppressive function in human osteosarcoma, vol. 14, pp. 16771-16781, 2015.

Dysregulation of microRNA (miR) is often associated with cancer development and progression. Aberrant expression of miR-134 has been found in some types of cancer. However, its expression and function in osteosarcoma remain unclear. The aim of this study was to explore the effects of miR-134 in osteosarcoma tumorigenesis and development. The expression level of miR-134 was quantified by real-time reverse transcription-polymerase chain reaction in human osteosarcoma cell lines and tissues.

Gene expression profile in breast cancer comprising predictive markers for metastatic risk

S. Sirirattanakul, Wannakrairot, P., Tencomnao, T., and Santiyanont, R., Gene expression profile in breast cancer comprising predictive markers for metastatic risk, vol. 14, pp. 10929-10936, 2015.

Quantitative multiplex reverse transcriptase-polymerase chain reaction was developed for the simultaneous detection of multiple-gene expression levels of formalin-fixed, paraffin-embedded breast cancer samples. Candidate genes were selected from previous microarray data relevant to breast cancer markers that had the potential to serve as predictive markers for metastatic risk. This multiplex gene set included 11 candidate and 3 housekeeping genes, and the aim was to predict breast cancer progression based on lymph node involvement status.

Correlation of EGFR gene amplification with invasion and metastasis of non-small cell lung cancer

X. F. Jia, Li, J., Zhao, H. B., Liu, J., and Liu, J. J., Correlation of EGFR gene amplification with invasion and metastasis of non-small cell lung cancer, vol. 14, pp. 11006-11012, 2015.

The aim of this study was to explore epidermal growth factor receptor (EGFR) gene amplification and its relationship with cancer invasion and metastasis in non-small cell lung cancer (NSCLC). EGFR amplification in 45 patients with NSCLC and 15 subjects with normal lung tissues was detected by fluorescence in situ hybridization. The relationship between EGFR amplification and the clinicopathologic features of NSCLC was analyzed.

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