Metagenomics

Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease

N. F. Rodrigues, Kästle, J., Coutinho, T. J. D., Amorim, A. T., Campos, G. B., Santos, V. M., Marques, L. M., Timenetsky, J., and de Farias, S. T., Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease, vol. 14, pp. 6518-6528, 2015.

The microbial community of the reproductive appara­tus, when known, can provide information about the health of the host. Metagenomics has been used to characterize and obtain genetic infor­mation about microbial communities in various environments and can relate certain diseases with changes in this community composition. In this study, samples of vaginal surface mucosal secretions were col­lected from five healthy cows and five cows that showed symptoms of reproductive disorders.

Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial diversity during cassava (Manihot esculenta crantz) fermentation

C. S. Kodama, Cuadros-Orellana, S., Bandeira, C. H. M. M., Graças, D. A., Santos, A. S., and Silva, A., Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial diversity during cassava (Manihot esculenta crantz) fermentation, vol. 13, pp. 1304-1313, 2014.

Denaturing high-performance liquid chromatography (DHPLC) has been described as a suitable method to study DNA polymorphisms. Here, cassava (Manihot esculenta Crantz) fermentation liquor was examined using DHPLC analysis to characterize the bacterial diversity during the fermentation process. GC-clamped amplicons corresponding to a variable region of the bacterial community 16S rDNA were synthesized using polymerase chain reaction (PCR) and then resolved on a base-composition basis using preparative DHPLC.

Composition and structure of microbial communities associated with different domestic sewage outfalls

Z. H. Wang, Yang, J. Q., Zhang, D. J., Zhou, J., Zhang, C. D., Su, X. R., and Li, T. W., Composition and structure of microbial communities associated with different domestic sewage outfalls, vol. 13, pp. 7542-7552, 2014.

The diversity of microbiota in waste waters has not been thoroughly examined, despite the potential impact of microbes on effluent quality. Wastewater microbial communities harbor pathogenic bacteria, viruses, and parasites. To study microbial communities in domestic sewage outfalls, 454 pyrosequencing technology was used to investigate the composition of microbial communities associated with municipal wastewater during different seasons sampled over the course of one year. A total of 195,103 16S rRNA gene sequences were obtained from 20 samples.

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