Metagenome

Isolation of a novel lipase from a metagenomic library derived from mangrove sediment from the south Brazilian coast

G. H. Couto, Glogauer, A., Faoro, H., Chubatsu, L. S., Souza, E. M., and Pedrosa, F. O., Isolation of a novel lipase from a metagenomic library derived from mangrove sediment from the south Brazilian coast, vol. 9, pp. 514-523, 2010.

A novel gene coding for a LipA-like lipase with 283 amino acids and a molecular mass of 32 kDa was isolated and characterized from a metagenomic library prepared from mangrove sediment from the south Brazilian coast. LipA was 52% identical to a lipolytic enzyme from an uncultured bacterium and shared only low identities (≤31%) with lipases/esterases from cultivable microorganisms.

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