Mesenchymal stem cells

Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality

R. V. Sampaio, Chiaratti, M. R., Santos, D. C. N., Bressan, F. F., Sangalli, J. R., Sá, A. L. A., Silva, T. V. G., Costa, N. N., Cordeiro, M. S., Santos, S. S. D., Ambrósio, C. E., Adona, P. R., Meirelles, F. V., Miranda, M. S., and Ohashi, O. M., “Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality”, vol. 14, pp. 53-62, 2015.

Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifying their capacity to proliferate.

Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay

L. C. Hermeto, Oliveira, R. J., Matuo, R., Jardim, P. H. A., DeRossi, R., Antoniolli, A. C. M. B., Deffune, E., Evaristo, T. C., and Santana, Á. E., “Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay”, vol. 14, pp. 339-348, 2015.

The use of mesenchymal stem cells (MSCs) in experimental, clinical, and therapeutic trials has grown in recent years. However, the issue remains of whether these procedures are completely safe for transplant patients. Therefore, this study was designed and carried out with the aim of evaluating two different comet assay protocols for genomic damage pattern analysis in MSCs derived from adipose tissue. The analyzed and interpreted results suggest that genetic testing is needed to support clonal expansion safety in cell therapy procedures with MSCs.

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