Matrix attachment region (MAR)

Characteristic element of matrix attachment region mediates vector attachment and enhances nerve growth factor expression in Chinese hamster ovary cells

X. Y. Wang, Zhang, J. H., Sun, Q. L., Yao, Z. Y., Deng, B. G., Guo, W. Y., Wang, L., Dong, W. H., Wang, F., Zhao, C. P., and Wang, T. Y., Characteristic element of matrix attachment region mediates vector attachment and enhances nerve growth factor expression in Chinese hamster ovary cells, vol. 14, pp. 9191-9199, 2015.

Preliminary studies have suggested that a characteristic element of the matrix attachment region (MAR) in human interferon-β mediates the adhesion of vectors to Chinese hamster ovary (CHO) cells. In this study, we investigated if vector adhesion increased nerve growth factor (NGF) expression in CHO cells. The MAR characteristic element sequence of human interferon-β was inserted into the multiple-cloning site of the pEGFP-C1 vector. The target NGF gene was inserted upstream of the MAR characteristic element sequence to construct the MAR/NGF expression vector.

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