Maternal plasma

Real-time PCR evaluation of cell-free DNA subjected to various storage and shipping conditions

Q. Wang, Cai, Y., Brady, P., and Vermeesch, J. R., Real-time PCR evaluation of cell-free DNA subjected to various storage and shipping conditions, vol. 14, pp. 12797-12804, 2015.

In this study, we attempted to explore the factors affecting the yield of cell-free fetal DNA (cffDNA) obtained from maternal blood samples, including the use of different types of collection tubes, the interval between sample processing, and sample shipping under extreme weather conditions. Blood samples were drawn into K3EDTA tubes and cell-stabilizing tubes (Streck blood collection tube, BCT) from women pregnant with male fetuses.

Detection of fetal RHD pseudogene (RHDΨ) and hybrid RHD-CE-Ds from RHD-negative pregnant women with a free DNA fetal kit

T. Gunel, Kalelioglu, I., Gedikbasi, A., Ermis, H., and Aydinli, K., Detection of fetal RHD pseudogene (RHDΨ) and hybrid RHD-CE-Ds from RHD-negative pregnant women with a free DNA fetal kit, vol. 10, pp. 2653-2657, 2011.

Hemolytic disease of the newborn is a clinical condition in which maternal and paternal Rh blood group antigens are incompatible and the mother is negative for the antigen whereas the father is positive. Analysis of fetal cells recovered from maternal plasma can provide a highly sensitive prenatal diagnosis. The fetal RHD gene in plasma DNA is detected by real-time PCR amplification of two different segments of the RHD gene (exons 7 and 10). Each amplicon is revealed with specific probes.

Subscribe to Maternal plasma