Marker transferability

Cross-amplification and characterization of microsatellite loci in Acropora austera from the south-western Indian Ocean

P. H. Montoya-Maya, Macdonald, A. H. H., and Schleyer, M. H., Cross-amplification and characterization of microsatellite loci in Acropora austera from the south-western Indian Ocean, vol. 13, pp. 1244-1250, 2014.

Here, we report the successful cross-species amplification of previously published acroporid microsatellite markers in the coral Acropora austera from the south-western Indian Ocean. This fast-growing species is a major reef-building coral on South African reefs; however, it is the most damaged coral by scuba diving activity, and is known to be very susceptible to coral bleaching. Neither genetic information nor symbiont-free host tissue was available to develop novel microsatellite markers for this species.

Characterization of nine transferred SSR markers in the tropical tree species Enterolobium contortisiliquum (Fabaceae)

P. A. Moreira, Sousa, S. A. S., Oliveira, F. A., Araújo, N. H., Fernandes, G. W., and Oliveira, D. A., Characterization of nine transferred SSR markers in the tropical tree species Enterolobium contortisiliquum (Fabaceae), vol. 11, pp. 3729-3734, 2012.

Transfer of molecular markers is widely used in conservation genetic studies. We investigated the transferability of simple sequence repeat (SSR) markers developed for Enterolobium cyclocarpum to E. contortisiliquum, a tropical tree widely distributed in dry forests. A set of 9 evaluated SSR markers were amplified in E. contortisiliquum and the degree of polymorphism was assessed in 8 trees sampled from each of 5 populations from central Brazil. All loci were polymorphic and the mean number of alleles for all loci was 6.

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