marker-assisted selection

Molecular detection of QTL controlling plant height components in a doubled haploid barley population

X. F. Ren, Sun, D. F., Dong, W. B., Sun, G. L., and Li, C. D., Molecular detection of QTL controlling plant height components in a doubled haploid barley population, vol. 13, pp. 3089-3099, 2014.

Yield losses caused by lodging in barley can be partially controlled by reducing plant height. In order to understand dwarfing mechanisms and efficiently use new dwarf germplasms for a breeding program, it is important to identify QTL of plant height components. QTL analysis was performed for seven plant height component traits using a DH population of 122 lines derived from the cross of Huaai 11 x Huadamai 6. Composite interval mapping procedures detected 20 QTL, which were mapped onto chromosomes 2H, 3H, 5H, 6H, and 7H.

A and MdMYB1 allele-specific markers controlling apple (Malus x domestica Borkh.) skin color and suitability for marker-assisted selection

X. J. Zhang, Wang, L. X., Chen, X. X., Liu, Y. L., Meng, R., Wang, Y. J., and Zhao, Z. Y., A and MdMYB1 allele-specific markers controlling apple (Malus x domestica Borkh.) skin color and suitability for marker-assisted selection, vol. 13, pp. 9103-9114, 2014.

Pre-selection for fruit skin color at the seedling stage would be highly advantageous, with marker-assisted selection offering a potential method for apple pre-selection. A and MdMYB1 alleles are allele-specific DNA markers that are potentially associated with apple skin color, and co-segregate with the Rf and Rni loci, respectively. Here, we assessed the potential application of these 2 alleles for marker-assisted breeding across 30 diverse cultivars and 2 apple seedling progenies.

Synergistic and threshold effects of GH1 and GHR promoter size variation on body growth and fat accrual in young Nelore (Bos indicus) bulls

M. A. Dani, von Cube, M., Freire, I. L., Suguisawa, L., Fischer, C., and Dani, S. U., Synergistic and threshold effects of GH1 and GHR promoter size variation on body growth and fat accrual in young Nelore (Bos indicus) bulls, vol. 11, pp. 1783-1798, 2012.

A synergistic effect in the somatotropic axis (GH1-GHR-IGF1) was observed in 736 young Nelore (Bos indicus) bulls under ad libitum grass feeding conditions on irrigated pasture in central Brazil. Stepwise substitution of shorter alleles of the promoter region of the growth hormone gene (GH1) and the P1 promoter of the GH1 receptor gene (GHR) with longer alleles was associated with significantly increased body weight gain (W550, weight at age 550 days; ADG, average daily gain) and fat accrual (FAT, rib eye fat thickness).

Identification of markers tightly linked to tomato yellow leaf curl disease and root-knot nematode resistance by multiplex PCR

S. X. Chen, Du, J. N., Hao, L. N., Wang, C. Y., Chen, Q., and Chang, Y. X., Identification of markers tightly linked to tomato yellow leaf curl disease and root-knot nematode resistance by multiplex PCR, vol. 11, pp. 2917-2928, 2012.

Seven different commercial F1 hybrids and two F2 populations were evaluated by multiplex PCR to identify plants that are homozygous or heterozygous for Ty-1 and Mi, which confer resistance to tomato yellow leaf curl disease and root-knot nematode, respectively. The Ty-1 and Mi markers were amplified by PCR and identified by digestion of the amplicons with the TaqI enzyme.

Updates and perspectives on the utilization of molecular makers of complex traits in rice

G. Li, Kwon, S. W., and Park, Y. J., Updates and perspectives on the utilization of molecular makers of complex traits in rice, vol. 11, pp. 4157-4168, 2012.

After complete sequencing of its genome and annotation of the majority of its ~32,000 genes, rice genome has become the model genome among the cereal genomes, and the focus has shifted from structural to functional genomics and application of genomic-derived information in rice breeding. During the past 2 decades, intensive worldwide efforts have led to significant improvements in rice.

Protein synthesis and degradation gene SNPs related to feed intake, feed efficiency, growth, and ultrasound carcass traits in Nellore cattle

R. C. Gomes, Silva, S. L., Carvalho, M. E., Rezende, F. M., Pinto, L. F. B., Santana, M. H. A., Stella, T. R., Meirelles, F. V., P. Júnior, R., Leme, P. R., and Ferraz, J. B. S., Protein synthesis and degradation gene SNPs related to feed intake, feed efficiency, growth, and ultrasound carcass traits in Nellore cattle, vol. 12, pp. 2923-2936, 2013.

We looked for possible associations of SNPs in genes related to protein turnover, with growth, feed efficiency and carcass traits in feedlot Nellore cattle. Purebred Nellore bulls and steers (N = 290; 378 ± 42 kg body weight, 23 months ± 42 days old) were evaluated for daily feed intake, body weight gain (BWG), gross feed efficiency, feed conversion ratio, partial efficiency of growth, residual feed intake (RFI), ultrasound backfat, rump fat, and ribeye area.

Marker-assisted selection in breeding silkworm strains with high silk production and resistance to the densonucleosis virus

C. X. Hou, Sun, P. J., Guo, X. J., Huang, Y. P., and Li, M. W., Marker-assisted selection in breeding silkworm strains with high silk production and resistance to the densonucleosis virus, vol. 12, pp. 4171-4178, 2013.

In the silkworm (Bombyx mori), resistance to the Zhenjiang (China) strain of the densonucleosis virus (DNV-Z) is controlled by the recessive gene nsd-Z (non-susceptible to DNV-Z), which is linked to 7 simple-sequence repeat markers. Marker-assisted evaluation and selection of DNV-Z-resistant silkworms were used for predicting DNV-resistance in backcrossed animals. A silkworm race was bred using this method, and its economic charac­teristics were found to be similar to those of commercial silkworm races.

A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat

W. L. Du, Wang, J., Wang, L. M., Pang, Y. H., Wu, J., Zhao, J. X., Yang, Q. H., and Chen, X. H., A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat, vol. 12, pp. 4797-4806, 2013.

In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequence-characterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search tool search showed that the sequence of pHs27 had no primary sequence homology with known sequences, and Southern blotting confirmed this result.

Incorporation of genetic marker information in beef cattle breeding programs

F. M. Rezende, Incorporation of genetic marker information in beef cattle breeding programs, vol. 12. pp. 5172-5173, 2013.

The availability of molecular marker information has turned out to be an opportunity to improve animal breeding programs, by the inclusion of those effects in the estimation of breeding values.

Molecular marker-assisted selection of the ae alleles in maize

F. Chen, Zhu, S. W., Xiang, Y., Jiang, H. Y., and Cheng, B. J., Molecular marker-assisted selection of the ae alleles in maize, vol. 9, pp. 1074-1084, 2010.

The ae (amylose extender) recessive mutant alleles in maize are an important genetic resource for the development of high-amylose cultivars. On the basis of ae allele sequences (from the National Center for Biotechnology Information), the ae mutant alleles were cloned from high-amylose maize and the allelic Ae gene from common maize luyuan92 inbred lines. Five pairs of primers were designed to screen for a molecular marker of ae alleles, yielding a dominant molecular marker, ae474.

Pages

Subscribe to marker-assisted selection