Marker assisted selection

Using SCC8, SCF27 and VMC7f2 markers in grapevine breeding for seedlessness via marker assisted selection

M. Akkurt, Çakır, A., Shidfar, M., Çelikkol, B. P., and Söylemezoğlu, G., Using SCC8, SCF27 and VMC7f2 markers in grapevine breeding for seedlessness via marker assisted selection, vol. 11, pp. 2288-2294, 2012.

We used molecular markers associated with seedlessness in grapes, namely SCC8, SCF27 and VMC7f2, to improve the efficiency of seedless grapevine breeding via marker assisted selection (MAS). DNA from 372 F1 hybrid progeny from the cross between seeded “Alphonse Lavallée” and seedless “Sultani” was amplified by PCR using three markers. After digestion of SCC8 marker amplification products by restriction enzyme BgIII, 40 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor (SdI) locus.

Evaluation of TFAM and FABP4 gene polymorphisms in three lines of Nellore cattle selected for growth

D. R. Ayres, Souza, F. R. P., Mercadante, M. E. Z., Fonseca, L. F. S., Tonhati, H., Cyrillo, J. N. S. G., Bonilha, S. F. M., and Albuquerque, L. G., Evaluation of TFAM and FABP4 gene polymorphisms in three lines of Nellore cattle selected for growth, vol. 9, pp. 2050-2059, 2010.

We analyzed the polymorphisms TFAM HaeIII, TFAM MboI and FABP4 MspA1I in three Nellore lines selected for growth in order to evaluate how selection affects the frequencies of these polymorphisms and evaluate their association with growth and carcass traits in Zebu cattle. Birth, weaning and yearling weights, rump height, longissimus muscle area, backfat thickness, and rump fat thickness were analyzed.

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