Mammals

Molecular conservation of the mammalian leptin protein

J. E. Gabriel and Lidani, K. C. F., “Molecular conservation of the mammalian leptin protein”, vol. 14. pp. 253-258, 2015.

In this study, we comparatively assessed multiple sequences of the leptin protein from different animal species to establish new insights into conservation degree of biological sequences and evolutionary biology among mammals using computational biology tools.

Limited efficiency of universal mini-barcode primers for DNA amplification from desert reptiles, birds and mammals

I. A. Arif, Khan, H. A., M. Sadoon, A., and Shobrak, M., “Limited efficiency of universal mini-barcode primers for DNA amplification from desert reptiles, birds and mammals”, vol. 10, pp. 3559-3564, 2011.

In recent years, DNA barcoding has emerged as a powerful tool for species identification. We report an extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds. Besides the standard double-annealing protocol, we also tested a more stringent single-annealing protocol. The PCR success rate for the amplification of the mini-barcode region was: mammals (4/5), reptiles (5/11) and birds (4/7).

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