Magnetic bead enrichment

Isolation and characterization of novel polymorphic microsatellite loci in Atrina vexillum Born (Pinnidae)

Y. S. Huang, Li, Q. H., Li, Z. B., Shangguan, J. B., Ning, Y. F., and Dai, G., “Isolation and characterization of novel polymorphic microsatellite loci in Atrina vexillum Born (Pinnidae)”, vol. 14, pp. 3541-3544, 2015.

The pen shell, Atrina vexillum Born, is an edible shellfish that is widely consumed in the Asia-Pacific region. In this study, 11 polymorphic microsatellite loci were isolated from A. vexillum, and 30 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 3 to 8. The polymorphism information content varied from 0.199 to 0.831. The observed and expected heterozygosities were 0.1000-0.8667 and 0.1244-0.8356, respectively.

Development and characterization of microsatellite loci in Megalonibea fusca

Z. B. Li, Li, Q. H., Ning, Y. F., Shangguan, J. B., Dai, G., Chen, L. N., Cao, Y. Y., and Chen, X. J., “Development and characterization of microsatellite loci in Megalonibea fusca”, vol. 14, pp. 4633-4636, 2015.

Megalonibea fusca is a commercially important large edible fish. In this study, the first set of 10 polymorphic microsatellite loci for M. fusca was developed and characterized. The number of alleles per locus ranged from two to five, with the observed and expected heterozygosities ranging from 0.0667 to 0.7667, and from 0.0644 to 0.5828, respectively. Most of the loci were in Hardy-Weinberg equilibrium (P > 0.05), except for two loci (Mf25 and Mf30) after a Bonferroni’s correction (P

Screening the first set of polymorphic microsatellite loci in Lunella coronata granulata (Turbinidae)

Z. B. Li, Li, Q. H., Shangguan, J. B., Ning, Y. F., and Dai, G., “Screening the first set of polymorphic microsatellite loci in Lunella coronata granulata (Turbinidae)”, vol. 14. pp. 6319-6322, 2015.

Lunella coronata granulata, from the family Turbini­dae, is an economically important species. The first set of 10 poly­morphic microsatellite loci was screened from L. coronata granulata, and 30 individuals were used to analyze the degree of polymorphism in these loci. The level of observed and expected heterozygosity was 0.0667-0.7333 and 0.0644-0.6628, respectively. The polymorphism information content varied from 0.305 to 0.559.

Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota

J. B. Shangguan, Li, Z. B., Ning, Y. F., Huang, Y. S., Yuan, Y., Lu, J., Li, B. B., and Mao, X. Q., “Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota”, vol. 14, pp. 6555-6560, 2015.

The sea cucumber Holothuria leucospilota has high me­dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilota were identified, and the relevant genetic variability index was assessed using 30 individu­als from a wild population.

Screening and characterization of new microsatellite markers in Fenneropenaeus penicillatus

J. B. Shangguan, Li, Z. B., Li, Q. H., Dai, G., and Ning, Y. F., “Screening and characterization of new microsatellite markers in Fenneropenaeus penicillatus”, vol. 13, pp. 6079-6082, 2014.

Fenneropenaeus penicillatus, with high protein and low fat, is a commercially important aquatic product in China. Microsatellite loci were developed according to the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats. Eight new polymorphic microsatellite markers for F. penicillatus were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The polymorphism information content ranged from 0.2703 to 0.7598, and the number of alleles per locus varied from 3 to 6.

Characterization of new microsatellite markers of Siganus fuscescens (Siganidae)

Q. H. Li, Li, Z. B., Dai, G., Chen, X. J., Chen, L. N., Cao, Y. Y., Shangguan, J. B., and Ning, Y. F., “Characterization of new microsatellite markers of Siganus fuscescens (Siganidae)”, vol. 12, pp. 2751-2754, 2013.

Siganus fuscescens, which is a small commercially important marine fish, is wildly distributed in shallow waters throughout the tropical and subtropical Indo-Pacific and Eastern Mediterranean regions. It is part of a group known as rabbitfish. Fifteen new polymorphic microsatellite markers for S. fuscescens were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 2 to 12, and the polymorphism information content ranged from 0.210 to 0.849.

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