Macrobrachium nipponense

Molecular characterization and upregulation of cytosolic manganese superoxide dismutase by imidazole derivative KK-42 in Macrobrachium nipponense

W. F. Wang, Mu, L. M., Wu, X. S., Yang, H., Ning, Q. J., Wang, W. F., Mu, L. M., Wu, X. S., Yang, H., and Ning, Q. J., Molecular characterization and upregulation of cytosolic manganese superoxide dismutase by imidazole derivative KK-42 in Macrobrachium nipponense, vol. 15, p. -, 2016.

Imidazole derivative KK-42 is a well-known regulator of insect growth. KK-42 pretreatment has been shown to promote the survival of Macrobrachium nipponense infected with Aeromonas hydrophila, possibly via activation of superoxide dismutase (SOD). In this study, the cytMnSOD gene was cloned from the hepatopancreas of M. nipponense using the rapid amplification of cDNA ends technique. The full-length cDNA of cytMnSOD was 1233 bp long, and the open reading frame was 858 bp long, encoding a 286-aa protein with a 60-aa leader sequence.

Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase

N. M. A. Rahman, Fu, H. T., Sun, S. M., Qiao, H., Jin, S., Bai, H. K., Zhang, W. Y., Liang, G. X., Gong, Y. S., Xiong, Y. W., Wu, Y., Rahman, N. M. A., Fu, H. T., Sun, S. M., Qiao, H., Jin, S., Bai, H. K., Zhang, W. Y., Liang, G. X., Gong, Y. S., Xiong, Y. W., and Wu, Y., Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase, vol. 15, p. -, 2016.

Nitric oxide synthase (NOS) produces nitric oxide (NO) by catalyzing the conversion of l-arginine to l-citrulline, with the concomitant oxidation of nicotinamide adenine dinucleotide phosphate. Recently, various studies have verified the importance of NOS invertebrates and invertebrates. However, the NOS gene family in the oriental river prawn Macrobrachium nipponense is poorly understood. In this study, we cloned the full-length NOS complementary DNA from M.

Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense

N. M. A. Rahman, Fu, H., Qiao, H., Jin, S., Bai, H., Zhang, W., Jiang, F. W., Liang, G., Sun, S., Gong, Y., Jiang, F. F., Xiong, Y., Wu, Y., Rahman, N. M. A., Fu, H., Qiao, H., Jin, S., Bai, H., Zhang, W., Jiang, F. W., Liang, G., Sun, S., Gong, Y., Jiang, F. F., Xiong, Y., and Wu, Y., Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense, vol. 15, p. -, 2016.

Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different developmental stages.

Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense

S. B. Jin, Fu, H. T., Jiang, S. F., Xiong, Y. W., Qiao, H., Zhang, W. Y., Gong, Y. S., and Wu, Y., Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense, vol. 14, pp. 18396-18406, 2015.

The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China. The androgenic gland produces hormones that play crucial roles in the differentiation of crustaceans to the male sex. MicroRNA (miRNA) post-transcriptionally regulates many protein-coding genes, influencing important biological and metabolic processes. However, currently, there is no published data identifying miRNA in M. nipponense. In this study, we identified novel miRNA in the androgenic gland of M. nipponense.

Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes

H. Qiao, Xiong, Y. W., Jiang, S. F., Fu, H. T., Sun, S. M., Jin, S. B., Gong, Y. S., and Zhang, W. Y., Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes, vol. 14, pp. 2041-2054, 2015.

This study utilized high-throughput RNA sequencing technology to identify reproduction- and development-related genes of Macrobrachium nipponense by analyzing gene expression profiles of testis and ovary. More than 20 million 1 x 51-bp reads were obtained by Illumina sequencing, generating more than 7.7 and 11.7 million clean reads in the testis and ovary library, respectively. As a result, 10,018 unitags were supposed to be differentially expressed genes (DEGs) between ovary and testis.

Characterization of the male-specific lethal 3 gene in the oriental river prawn, Macrobrachium nipponense

Y. P. Zhang, Sun, S. M., Fu, H. T., Ge, X. P., Qiao, H., Zhang, W. Y., Xiong, Y. W., Jiang, S. F., Gong, Y. S., and Jin, S. B., Characterization of the male-specific lethal 3 gene in the oriental river prawn, Macrobrachium nipponense, vol. 14, pp. 3106-3120, 2015.

In this study, male-specific lethal 3 homolog (Mnmsl3) was cloned and characterized from the freshwater prawn Macrobrachium nipponense (Crustacea: Decapoda: Palaemonidae) by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnmsl3 showed high-sequence homology to the insect Msl3 and contained a conserved chromatin organization modifier domain and an MORF4-related gene domain.

Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense

W. Y. Zhang, Jiang, S. F., Xiong, Y. W., Fu, H. T., Qiao, H., Sun, S. M., Gong, Y. S., and Jin, S. B., Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense, vol. 14, pp. 4318-4330, 2015.

The gene female sterile homeotic (fsh) plays crucial roles in molecular function, including protein kinase activity and DNA binding, which are involved in biological processes such as terminal region determination and negative regulation of DNA-dependent transcription. Although fsh has been found in Drosophila melanogaster, little is known regarding its expression in crustaceans.

Molecular cloning, characterization, and expression analysis of a Broad-Complex homolog during development in the oriental river prawn Macrobrachium nipponense

S. F. Jiang, Zhang, Y. P., Sun, S. M., Gong, Y. S., Xiong, Y. W., Qiao, H., Zhang, W. Y., Jin, S. B., and Fu, H. T., Molecular cloning, characterization, and expression analysis of a Broad-Complex homolog during development in the oriental river prawn Macrobrachium nipponense, vol. 14, pp. 5141-5152, 2015.

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors. In this study, we isolated the full-length cDNA of a BR-C homolog from the testes of the oriental river prawn (Macrobrachium nipponense), according to established expressed sequence tag information, using the rapid amplification of cDNA ends technique. The homolog was designated as MnBR-C. The full-length cDNA of MnBR-C contained a 1095-bp open reading frame encoding a precursor protein of 365 amino acid residues.

Identification of spliced mRNA isoforms of retinoid X receptor (RXR) in the Oriental freshwater prawn Macrobrachium nipponense

Z. Li, Wang, W. Q., Zhang, E. F., and Qiu, G. F., Identification of spliced mRNA isoforms of retinoid X receptor (RXR) in the Oriental freshwater prawn Macrobrachium nipponense, vol. 13, pp. 3914-3926, 2014.

Retinoid X receptors (RXR) are members of the nuclear receptor family that are conserved from invertebrates to vertebrates, and they play an essential role in regulating reproductive maturation, molting, and embryo development. In this study, five RXR isoforms, named RXRL2 (L, long form), RXRL3, RXRS1 (S, short form), RXRS2, and RXRS3, containing six domains from A to F, were cloned from the prawn Macrobrachium nipponense using 5ꞌ- and 3ꞌ- rapid amplification of cDNA ends.

Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense

Y. P. Zhang, Qiao, H., Zhang, W. Y., Sun, S. M., Jiang, S. F., Gong, Y. S., Xiong, Y. W., Jin, S. B., and Fu, H. T., Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense, vol. 12, pp. 4698-4711, 2013.

In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned and characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnsxl1 and Mnsxl2 showed high sequence homology to the insect Sxl and contained conserved domains in two RNA-binding motifs.

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