M2 macrophage

Interleukin-4 regulates macrophage polarization via the MAPK signaling pathway to protect against atherosclerosis

X. N. Zhao, Li, Y. N., Wang, Y. T., Zhao, X. N., Li, Y. N., and Wang, Y. T., Interleukin-4 regulates macrophage polarization via the MAPK signaling pathway to protect against atherosclerosis, vol. 15, p. -, 2016.

Our study aimed to investigate the effects of interleukin-4 (IL-4) on macrophage polarization, as well as its role in the development of atherosclerosis. Human peripheral blood mononuclear cells (PBMCs) were isolated and randomly divided into 3 groups: control group, ox-LDL group, and ox-LDL + IL-4 groups. The expression of M1/M2 macrophage surface markers such as TNF-α, CD68, and CD206 were analyzed by western blot. Cell viability was determined using the MTT assay. Measurement of CD86/CD206 expression ratio (M1/M2 ratio) was performed via flow cytometry.

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