Lung

Comparison of three methods of DNA extraction from peripheral blood mononuclear cells and lung fragments of equines

E. M. Santos, Paula, J. F. R., Motta, P. M. C., Heinemann, M. B., Leite, R. C., Haddad, J. P. A., Del Puerto, H. L., and Reis, J. K. P., Comparison of three methods of DNA extraction from peripheral blood mononuclear cells and lung fragments of equines, vol. 9. pp. 1591-1598, 2010.

We compared three different protocols for DNA extraction from horse peripheral blood mononuclear cells (PBMC) and lung fragments, determining average final DNA concentration, purity, percentage of PCR amplification using β-actin, and cost. Thirty-four samples from PBMC, and 33 samples from lung fragments were submitted to DNA extraction by three different protocols. Protocol A consisted of a phenol-chloroform and isoamylic alcohol extraction, Protocol B used alkaline extraction with NaOH, and Protocol C used the DNAzol® reagent kit.

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