LPS

Quercetin ameliorates LPS-induced inflammation in human peripheral blood mononuclear cells by inhibition of the TLR2-NF-κB pathway

M. Zhang, Lin, J. M., Li, X. S., Li, J., Zhang, M., Lin, J. M., Li, X. S., and Li, J., “Quercetin ameliorates LPS-induced inflammation in human peripheral blood mononuclear cells by inhibition of the TLR2-NF-κB pathway”, vol. 15, p. -, 2016.

Quercetin, a dietary flavonoid abundant in fruits, vegetables, and herbs, presents various pharmacological effects. This study aimed to investigate the anti-inflammatory effect and the underlying mechanism of quercetin in lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMCs). Cell viability was measured by the Cell Counting Kit-8 assay. The mRNA expression of Toll-like receptor 2 (TLR2) was assessed by quantitative real-time polymerase chain reaction.

Study of the expression of GABAA receptor in rats during acute lung injury caused by endotoxin

L. Y. Zhan, Du, L., Xia, Z. Y., Li, W. L., and Zhao, B., “Study of the expression of GABAA receptor in rats during acute lung injury caused by endotoxin”, vol. 14, pp. 13312-13319, 2015.

The objective of the present study was to investigate the role of γ-aminobutyric acid type A receptor (GABAAR) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats. Thirty-two male wistar rats were randomly divided into four groups. Rats in the GABA group were pretreated with LPS and GABA, while those in the bicuculline (BIC) group were pretreated with LPS and bicuculline. We assessed the arterial blood gas, dry/wet ratio, and the level of tumor necrosis factor-α (TNF-α), IL-6, malondialdehyde, and superoxide dismutase 6 h after the immunization.

Identification of genes and pathways related to lipopolysaccharide signaling in duckling spleens

B. Deng, Yu, T., Liu, W., Ye, S. Q., Wang, L. X., Yang, Y., Gong, P., Ran, Z. P., Huang, H. J., and Wen, J. H., “Identification of genes and pathways related to lipopolysaccharide signaling in duckling spleens”, vol. 14, pp. 17312-17321, 2015.

Lipopolysaccharide (LPS), the major component of the outer cell wall of Gram-negative bacteria, activates the immune system and threatens the health of livestock and poultry. However, little is known about the genes and pathways involved in the immune response of ducklings to LPS. To elucidate the genes involved in the response of 7-day-old duckling spleens treated with LPS, RNA from LPS-treated and control duckling spleens was analyzed by RNA-Seq. The results showed 11,095 and 10,840 genes with >10 clean reads in the LPS-treated and control groups, respectively.

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