Linker

Linker length affects expression and bioactivity of the onconase fusion protein in Pichia pastoris

G. G. Yang, Xu, X. Y., Ding, Y., Cui, Q. Q., Wang, Z., Zhang, Q. Y., Shi, S. H., Lv, Z. Y., Wang, X. Y., Zhang, J. H., Zhang, R. G., and Xu, C. S., “Linker length affects expression and bioactivity of the onconase fusion protein in Pichia pastoris”, vol. 14, pp. 19360-19370, 2015.

The aim of this study was to analyze the effect of linker length on the expression and biological activity of recombinant protein onconase (ONC) in fusion with human serum albumin (HSA) in Pichia pastoris. Four flexible linkers with different lengths namely Linker L0, L1: (GGGGS)1, L2: (GGGGS)2, and L3:(GGGGS)3 were inserted into the fusion gene and referred to as HSA-n-ONC, where N = 0, 5, 10, or 15. The sequence of the fusion gene HSA-ONC was designed based on the GC content and codon bias in P.

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