Ligase detection reaction

Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness

J. T. Shu, Zhang, M., Shan, Y. J., Xu, W. J., Chen, K. W., and Li, H. F., Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness, vol. 14, pp. 1393-1403, 2015.

The micromolar calcium-activated neutral protease gene (CAPN1) is a physiological candidate gene for meat tenderness. Four previously identified single nucleotide polymorphism (SNP) markers located within the CAPN1 gene were evaluated for their associations with variation in the meat tenderness of a Chinese indigenous chicken breed, a higher meat quality breed (i.e., Qingyuan partridge chicken), and the commercial Recessive White chicken breed.

Transcriptional co-activator PGC-1α gene is associated with chicken skeletal muscle fiber types

J. T. Shu, Xu, W. J., Zhang, M., Song, W. T., Shan, Y. J., Song, C., Zhu, W. Q., Zhang, X. Y., and Li, H. F., Transcriptional co-activator PGC-1α gene is associated with chicken skeletal muscle fiber types, vol. 13, pp. 895-905, 2014.

The peroxisome proliferators-activated receptor-γ coactivator-1α (PGC-1α) is a candidate gene for meat quality traits because of its prominent role in muscle fiber type switching and determination. We investigated the effects of the PGC-1α gene on chicken skeletal muscle fiber type switching and on other meat quality traits. Single nucleotide polymorphisms were detected by PCR-SSCP and DNA sequencing, and then genotyping was performed by PCR-ligation detection reaction methods.

Association of chicken growth hormone polymorphisms with egg production

Y. J. Su, Shu, J. T., Zhang, M., Zhang, X. Y., Shan, Y. J., Li, G. H., Yin, J. M., Song, W. T., Li, H. F., and Zhao, G. P., Association of chicken growth hormone polymorphisms with egg production, vol. 13, pp. 4893-4903, 2014.

Growth hormone (GH) has diverse functions in animals, together with other hormones from the somatotropic axis. Here, chicken GH (cGH) was investigated in recessive white chickens and Qingyuan partridge chickens as a candidate gene affecting egg production traits. Chicken egg production traits were studied in association with 4 selected single nucleotide polymorphisms (T185G, G662A, T3094C, and C3199T). Genotyping was performed by the polymerase chain reaction-ligase detection reaction method.

Subscribe to Ligase detection reaction