Leukocyte

Analysis of blood processing conditions to obtain high-quality total RNA from human leukocyte concentrate

I. A. G. Pérez, Santana, S. P., Argudin, T. D., and Gardon, D. O. P., Analysis of blood processing conditions to obtain high-quality total RNA from human leukocyte concentrate, vol. 6, pp. 298-307, 2007.

Blood samples are used as a biological source to discover biomarkers of hematological and non-hematological disorders. The present study shows the impact of different experimental conditions associated with cell lysis buffer, TRI-reagent protocol and blood cell storage buffer and their correlation with the quantity, quality and Adrenomedullin gene expression levels of total RNA when RT-PCR technique is used. A leukocyte cell bank protocol is also proposed for further mRNA expression analysis using RNAlater as storage buffer.

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