Leukemia inhibitory factor

Effects of modified Shoutaiwai recipe on integrin β3 and leukemia-inhibitory factor in endometrium of controlled ovarian hyperstimulation mice during the implantation window

X. Y. Chen, Chen, J., Wang, Z. Y., Yu, X. H., Wei, B. X., and Wu, X. H., Effects of modified Shoutaiwai recipe on integrin β3 and leukemia-inhibitory factor in endometrium of controlled ovarian hyperstimulation mice during the implantation window, vol. 14, pp. 2970-2977, 2015.

We investigated the effects of a modified Shoutaiwai recipe on integrin β3 and leukemia-inhibitory factor (LIF) in the endometrium of controlled ovarian hyperstimulation (COH) mice during the implantation window. Seventy non-pregnant mice were randomly divided into 3 groups: a traditional medicine (TCM) treatment group (N = 30), an aspirin treatment (N = 30) group, and a control group (N = 10).

Construction and identification of pIRES2-LIF-NT-3 bicistronic eukaryotic expression vector

B. N. Li, Li, W. D., Lin, J. T., Feng, H. G., and Yuan, Z. Q., Construction and identification of pIRES2-LIF-NT-3 bicistronic eukaryotic expression vector, vol. 13, pp. 4691-4703, 2014.

We used a simple and efficient method to construct a bicistronic eukaryotic expression vector pIRES2-LIF-NT-3. The leukemia inhibitory factor (LIF) and neurotrophin-3 (NT-3) genes were obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The LIF cDNA fragment was inserted into the multiple cloning sites of a vector containing internal ribosome entry site and enhanced green fluorescent protein (EGFP) (pIRES2-EGFP) to generate the bicistronic eukaryotic expression plasmid pIRES2-LIF-EGFP.

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