Laminin

Selection and validation of reference house­keeping genes in the J774A1 macrophage cell line for quantitative real-time PCR

F. B. Ferraz, Fernandez, J. H., Ferraz, F. B., and Fernandez, J. H., Selection and validation of reference house­keeping genes in the J774A1 macrophage cell line for quantitative real-time PCR, vol. 15, p. -, 2016.

Macrophages are essential components of the innate and adaptive immune responses, playing a decisive role in atherosclerosis, asthma, obesity, and cancer. The differential gene expression resulting from adhesion of macrophages to the extra-cellular matrix (ECM) has been studied in the J774A1 murine macrophage cell line using quantitative polymerase chain reaction (qPCR). The goal of this study was to identify housekeeping genes (HKGs) that remain stable and unaltered under normal culture conditions and in the presence of laminin after a time lapse of 6 and 24 h.

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