Simplified preparation of a DNA ladder using PCR

T. - Y. Wang, Wang, L., and Wang, F., Simplified preparation of a DNA ladder using PCR, vol. 10. pp. 1631-1635, 2011.

Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol and mixing. Fragments of 100- to 1000-bp DNA were successfully amplified; the sequences showed 100% identity with lambda DNA.

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