Kidney

Effects of autologous SCF- and G-CSF-mobilized bone marrow stem cells on hypoxia-inducible factor-1 in rats with ischemia-reperfusion renal injury

L. Y. Bi, Zhao, D. A., Yang, D. S., Guo, J. G., Liang, B., Zhang, R. X., Zhao, J. L., Bai, H. T., and Li, S. J., Effects of autologous SCF- and G-CSF-mobilized bone marrow stem cells on hypoxia-inducible factor-1 in rats with ischemia-reperfusion renal injury, vol. 14, pp. 4102-4112, 2015.

To explore the mechanism whereby stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) jointly mobilize bone marrow stem cells (BMSCs) and promote kidney repair, male Sprague-Dawley rats were randomly assigned into 4 groups. In the treatment control group, rats were administered SCF (200 μg·kg-1·day-1) and G-CSF (50 μg·kg-1·day-1) for 5 days. In the treatment group, RIRI models were established, and 6 h later, SCF (200 μg·kg-1·day-1) and G-CSF (50 μg·kg-1·day-1) were administered for 5 days.

Regulation of WNK4 gene transcription in the kidneys

Z. J. Sun, Li, Y., Li, Y. H., Zhang, J. S., Ding, Q., Li-Ling, J., Liang, Y., and Zhao, Y. Y., Regulation of WNK4 gene transcription in the kidneys, vol. 12, pp. 2332-2340, 2013.

With-no-lysine (K) kinase-4 (WNK4) is a newly cloned kinase-encoding gene that plays a crucial role in the maintenance of electrolyte homeostasis. Mutations of WNK4 can cause pseudohypoal­dosteronism type α, an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension. We explored the expression and regulatory mechanism of WNK4 in the human kidneys, which is a key regulator of blood pressure. Expression of WNK4 was determined by RT-PCR.

Expression of 1alpha-HYD and 24-HYD in bovine kidney mediated by vitamin D3 supplementation

L. R. Rezende, Delgado, E. F., Júnior, A. R. L., Gasparin, G., Jorge, E. C., Mourão, G. B., and Coutinho, L. L., Expression of 1alpha-HYD and 24-HYD in bovine kidney mediated by vitamin D3 supplementation, vol. 12, pp. 6611-6618, 2013.

In order to better understand vitamin D3 in cattle metabolism, we quantified 1alpha-HYD and 24-HYD gene expression. In the kidneys of 35 male Nellore cattle, these were divided into a control group and two treatment groups (2 x 106 international units of vitamin D3 administered for 2 or 8 consecutive days pre-slaughter).

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