Karyomorphology

Karyotype variation in cultivars and spontaneous cocoa mutants (Theobroma cacao L.)

G. S. F. Figueiredo, Melo, C. A. F., Souza, M. M., Araújo, I. S., Zaidan, H. A., Pires, J. L., and Ahnert, D., Karyotype variation in cultivars and spontaneous cocoa mutants (Theobroma cacao L.), vol. 12, pp. 4667-4677, 2013.

Four mutant cocoa accessions with morphological changes and a cultivar sample were karyomorphologically characterized. Slides were prepared by enzymatic digestion of the root meristem and squashed in 45% acetic acid, followed by 2% Giemsa staining. The chromosome number of 2n = 20 was seen in all accessions. The karyotype formula for Cacau Comum and Cacau Rui was 2n = 20m. Submetacentric chromosomes were observed in Cacau Pucala and Cacau Jaca, both with 2n = 18m + 2sm, but the karyotype formula for Cacau Sem Vidro was 2n = 16m + 4sm.

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