ITS2

A tiered barcode authentication tool to differentiate medicinal Cassia species in India

N. Purushothaman, Newmaster, S. G., Ragupathy, S., Stalin, N., Suresh, D., Arunraj, D. R., Gnanasekaran, G., Vassou, S. L., Narasimhan, D., and Parani, M., A tiered barcode authentication tool to differentiate medicinal Cassia species in India, vol. 13, pp. 2959-2968, 2014.

DNA barcoding is a desirable tool for medicinal product authentication. DNA barcoding is a method for species identification using short DNA sequences that are conserved within species, but variable between species. Unlike animals, there is no single universal DNA barcode locus for plants. Coding markers, matK and rbcL, and noncoding markers, trnH-psbA (chloroplast) and ITS2 (nuclear), have been reported to be suitable for the DNA barcoding of plants with varying degree of success.

Barcoding the major Mediterranean leguminous crops by combining universal chloroplast and nuclear DNA sequence targets

P. Madesis, Ganopoulos, I., Ralli, P., and Tsaftaris, A., Barcoding the major Mediterranean leguminous crops by combining universal chloroplast and nuclear DNA sequence targets, vol. 11, pp. 2548-2558, 2012.

The ability to discriminate all species is the ultimate target in barcoding. The Mediterranean basin is a center of origin for legumes and thus they have played a key role in feeding the Mediterranean population. It is also a region with important protected designation of origin and protected geographical indication legumes that provide income in rural areas. We evaluated the use of two chloroplast regions, trnL and rpoC1, and a nuclear internal transcriber region, ITS2, for their efficiency to barcode the main Mediterranean leguminous crops.

DNA barcoding of populations of Fallopia multiflora, an indigenous herb in China

X. Q. Sun, Bai, M. M., Yao, H., Guo, J. L., Li, M. M., and Hang, Y. Y., DNA barcoding of populations of Fallopia multiflora, an indigenous herb in China, vol. 12, pp. 4078-4089, 2013.

Fallopia multiflora, locally known as Heshouwu, is one of the most important and widely used Chinese medicinal herbs. However, there is still considerable confusion concerning its different provenances. DNA barcoding is a recent aid to taxonomic identification and uses a short standardized DNA region to discriminate plant species. We assessed the applicability of 4 candidate DNA barcodes (matK, rbcL, psbA-trnH, and ITS2) to identify populations of F. multiflora.

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