ITS

Development of genetic markers for Ochradenus arabicus (Resedaceae), an endemic medicinal plant of Saudi Arabia

S. Khan, Al-Qurainy, F., Nadeem, M., and Tarroum, M., “Development of genetic markers for Ochradenus arabicus (Resedaceae), an endemic medicinal plant of Saudi Arabia”, vol. 11, pp. 1300-1308, 2012.

Some species of the genus Ochradenus are difficult to identify based on morphological markers. Similar limitations are found for biochemical markers. We developed genetic markers based on DNA sequences for Ochradenus arabicus, which is an endemic plant to Saudi Arabia, locally utilized as a medicinal shrub. The internal transcribed spacer sequence of nuclear ribosomal DNA and chloroplast (rpoB and rpoC1) markers were more informative than other chloroplast DNA markers.

Molecular phylogenetic study of Cardamine amaraeformis Nakai using nuclear and chloroplast DNA markers

M. A. Ali, Lee, J., Kim, S. Y., and Al-Hemaid, F. M. A., “Molecular phylogenetic study of Cardamine amaraeformis Nakai using nuclear and chloroplast DNA markers”, vol. 11, pp. 3086-3090, 2012.

The internal transcribed spacer regions of nuclear ribosomal DNA, trnL and trnL-F chloroplast genes of Cardamine amaraeformis Nakai (Brassicaceae) were sequenced and analyzed with the sequences of related Cardamine sp available in GenBank to detect the pattern of C. amaraeformis evolutionary differentiation. C.

Phylogeny and systematics of Indian Polygonum sensu lato in the subfamily Polygonoideae based on ITS sequences of nuclear ribosomal DNA

R. K. Choudhary, Park, S. - H., and Lee, J., “Phylogeny and systematics of Indian Polygonum sensu lato in the subfamily Polygonoideae based on ITS sequences of nuclear ribosomal DNA”, vol. 11, pp. 4370-4382, 2012.

The nuclear ribosomal DNA internal transcribed spacer (ITS) sequences from 44 Indian Polygonum taxa were examined to investigate relationships among various sections proposed by earlier researchers. The maximum parsimony trees obtained from analysis of the ITS sequences suggested eight major groups of the Indian Polygonum spp. The relationships among different sections were largely congruent with those inferred from morphological characters as described by Hooker.

Genetic variability in the ITS and IGS regions of the ribosomal DNA of Acremonium cavaraeanum exhibiting antimicrobial activity

A. R. Todaro, Nascimento, V. X., Souza, N. C. C., Silva, P. P., Santos, J. M., Ramalho, E. A. V. F., Melo, T. V. C., Silva, I. S. M., Júnior, G. S. Lins, Bastos, M. L. A., Farias, D. F., Souza, P. C. V., Carvalho, A. F. U., Silva, M. C. S., Almeida, R. M. R. G., and E. Neto, R., “Genetic variability in the ITS and IGS regions of the ribosomal DNA of Acremonium cavaraeanum exhibiting antimicrobial activity”, vol. 12, pp. 6983-6995, 2013.

Endophytic microorganisms represent promising alternatives for obtaining new drugs of biotechnological importance. In this study, the endophytic species Acremonium cavaraeanum (A1a) isolated from Cocos nucifera was cultivated for the production of secondary metabolites, and its extracts and fractions were evaluated by the dilution method (MIC). The EtOAc extracts and MeOH fractions were tested against Gram-positive and -negative bacteria, and had an MIC of 125 μg/mL when evaluated in the EtOAc extract (EBI).

Subscribe to ITS