ISSR-PCR

Genetic relationship among Labisia pumila (Myrsinaceae) species based on ISSR-PCR

E. Karimi, Jaafar, H. Z. E., Aziz, M. A., Taheri, S., and AzadiGonbad, R., Genetic relationship among Labisia pumila (Myrsinaceae) species based on ISSR-PCR, vol. 13, pp. 3301-3309, 2014.

The genus Labisia (Myrsinaceae) is a popular medicinal plant in Malaysia. We examined the genetic relationship among three varieties of L. pumila var. pumila, L. pumila var. alata, L. pumila var. lanceolata and Labisia paucifolia using an ISSR assay. Fifty-eight primers were tested, among which 18 gave reliable polymorphic banding patterns; these yielded 264 polymorphic markers.

Genetic relationships among five varieties of Curcuma alismatifolia (Zingiberaceae) based on ISSR markers

S. Taheri, Abdullah, T. L., Abdullah, N. A. P., and Ahmad, Z., Genetic relationships among five varieties of Curcuma alismatifolia (Zingiberaceae) based on ISSR markers, vol. 11, pp. 3069-3076, 2012.

The genus Curcuma is a member of the ginger family (Zingiberaceae) that has recently become popular for use as flowering pot plants, both indoors and as patio and landscape plants. We used PCR-based molecular markers (ISSRs) to assess genetic variation and relationships between five varieties of curcuma (Curcuma alismatifolia) cultivated in Malaysia. Sixteen ISSR primers generated 139 amplified fragments, of which 77% had high polymorphism among these varieties.

Genetic diversity of Phytophthora capsici (Pythiaceae) isolates in Anhui Province of China based on ISSR-PCR markers

P. Li, Cao, S., Dai, Y. L., Li, X. L., Xu, D. F., Guo, M., Pan, Y. M., and Gao, Z. M., Genetic diversity of Phytophthora capsici (Pythiaceae) isolates in Anhui Province of China based on ISSR-PCR markers, vol. 11, pp. 4285-4296, 2012.

Phytophthora capsici is a plant pathogenic oomycete that damages numerous crops worldwide. Consequently, interest in research on the genetic structure of this species has grown in recent decades. However, there is little information about P. capsici in eastern China. We investigated the genetic diversity of P. capsici isolates from three large regions of Anhui Province in eastern China based on ISSR-PCR technology. Thirteen random primers were screened and used to amplify DNA from 51 samples.

Characterization of Chinese eggplant isolates of the fungal pathogen Verticillium dahliae from different geographic origins

Z. Xu, Ali, Z., Hou, X., Li, H., Yi, J. X., and Abbasi, P. A., Characterization of Chinese eggplant isolates of the fungal pathogen Verticillium dahliae from different geographic origins, vol. 12, pp. 183-195, 2013.

Verticillium dahliae is a fungal pathogen that causes wilt disease in a wide range of host plants. Characterization of virulence, morphological, and molecular variations among V. dahliae isolates from different geographic origins is essential for any breeding program aimed at producing plant cultivars resistant to this disease. We characterized virulence variation among V.

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