Isozymes

Correlation of genetic variation among wild Trigonella foenum-graecum L. accessions with their antioxidant potential status

E. A. Haliem and Al-Huqail, A. A., Correlation of genetic variation among wild Trigonella foenum-graecum L. accessions with their antioxidant potential status, vol. 13, pp. 10464-10481, 2014.

In this study, we analyzed the correlation between genetic variation based on random amplified polymorphic DNA (RAPD), acid phosphatase, and glutamate-oxaloacetate transaminase isozymes, and amino acid composition with the antioxidant potential status of 7 wild Trigonella foenum-graecum L. accessions collected from diverse ecogeographical regions. RAPD revealed that 90 DNA products had highly polymorphism value (94.12%) based on band numbers, with sizes ranging from 50-2100 base pairs, and band intensity.

Genetic diversity analysis of Capsicum spp germplasm bank accessions based on α/β-esterase polymorphism

E. R. Monteiro, Bronzato, A. R., Orasmo, G. R., Lopes, A. C. A., Gomes, R. L. F., Mangolin, C. A., and Machado, M. F. P. S., Genetic diversity analysis of Capsicum spp germplasm bank accessions based on α/β-esterase polymorphism, vol. 12, pp. 1155-1167, 2013.

Genetic diversity and structure were analyzed in 10 accessions belonging to Banco Ativo de Germoplasma de Capsicum located at Federal University of Piauí in northwestern Brazil that receives pepper samples grown in community gardens in various regions and Brazilian states. Selections were made from seeds of C. chinense (4 accessions), C. annuum (5 accessions), and C. baccatum (1 accession). Samples consisting of leaves were collected from 4-10 plants of each accession (a total of 85 plants).

Multiple Mantel tests and isolation-by-distance, taking into account long-term historical divergence

M. Pires de C. Telles and Diniz-Filho, J. Alexandre, Multiple Mantel tests and isolation-by-distance, taking into account long-term historical divergence, vol. 4. pp. 742-748, 2005.

Mantel tests of matrix correspondence have been widely used in population genetics to examine microevolutionary processes, such as isolation-by-distance (IBD). We used partial and multiple Mantel tests to simultaneously test long-term historical effects and current divergence and equilibrium processes, such as IBD. We used these procedures to calculate genetic divergence among Eugenia dysenterica (Myrtaceae) populations in Central Brazil.

Genetic diversity of Brazilian natural populations of Anthonomus grandis Boheman (Coleoptera: Curculionidae), the major cotton pest in the New World

W. F. S. Martins, Ayres, C. F. J., and Lucena, W. A., Genetic diversity of Brazilian natural populations of Anthonomus grandis Boheman (Coleoptera: Curculionidae), the major cotton pest in the New World, vol. 6, pp. 23-32, 2007.

Twenty-five RAPD loci and 6 isozyme loci were studied to characterize the genetic variability of natural populations of Anthonomus grandis from two agroecosystems of Brazil. The random-amplified polymorphic DNA data disclosed a polymorphism that varied from 52 to 84% and a heterozygosity of 0.189 to 0.347. The index of genetic differentiation (GST) among the six populations was 0.258. The analysis of isozymes showed a polymorphism and a heterozygosity ranging from 25 to 100% and 0.174 to 0.277, respectively.

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