Isolation and analysis of cell-free fetal DNA from maternal peripheral blood in Chinese women
“Isolation and analysis of cell-free fetal DNA from maternal peripheral blood in Chinese women”, vol. 14, pp. 18078-18089, 2015.
, Non-invasive prenatal diagnosis is used to detect the genetic material of the fetus by isolating the cell-free fetal DNA (cffDNA) from maternal peripheral blood. In order to establish an isolation method for cffDNA from maternal peripheral blood in Chinese women, the cffDNA was acquired with a two-step centrifugation using a QlAamp DNA Blood mini kit. The SRY gene of plasma DNA was amplified by polymerase chain reaction (PCR). Real-time quantitative PCR was used to measure the concentration of cffDNA in maternal peripheral blood in different pregnant women.