Intervertebral disc degeneration

Effect of the mixture of bone marrow mesenchymal stromal cells and annulus fibrosus cells in repairing the degenerative discs of rabbits

Y. H. Wang, Yang, B., Li, W. L., and Li, J. M., Effect of the mixture of bone marrow mesenchymal stromal cells and annulus fibrosus cells in repairing the degenerative discs of rabbits, vol. 14, pp. 2365-2373, 2015.

The aims of this study were to investigate the effect of a mixture of bone marrow mesenchymal stem cells (BMSCs) and annulus fibrosus cells (AFCs) in repairing the degenerative discs of rabbits, and to provide an experimental basis for its clinical application. BMSCs from rabbits were cultured in vitro and mixed with AFCs. The animal model of degenerative intervertebral disc was built by aspirating the nucleus pulposus (L3-4, L4-5, L5-6, and L6-7) through a posterolateral approach.

Co-culture of annulus fibrosus cells and bone marrow mesenchymal stem cells

Y. Xu, Zhang, X. J., Fang, L., and Zhao, T. B., Co-culture of annulus fibrosus cells and bone marrow mesenchymal stem cells, vol. 14, pp. 3932-3938, 2015.

To investigate the effect on cell proliferation and extracellular matrix expression of annulus fibrosus (AF) cells when co-cultured with bone marrow mesenchymal stem cells (BMSCs). Primary isolated rabbit BMSCs and AF cells were cultured and harvested cells were placed into a 15-mL centrifugal tube co-culture system in a ratio of 2:1 (A group), 1:1 (B group), and 1:2 (C group). Cell proliferation was evaluated using cell counting kit-8, and mRNA of collagen II and mucopolysaccharide was quantified using real-time polymerase chain reaction (PCR) on Days 7, 14, and 21.

Association between VEGF and eNOS gene polymorphisms and lumbar disc degeneration in a young Korean population

I. B. Han, Ropper, A. E., Teng, Y. D., Shin, D. A., Jeon, Y. J., Park, H. M., Shin, D. E., Park, Y. S., Kim, K. N., and Kim, N. - K., Association between VEGF and eNOS gene polymorphisms and lumbar disc degeneration in a young Korean population, vol. 12, pp. 2294-2305, 2013.

Disturbances in blood flow to intervertebral discs (IVD) play an important role in IVD degeneration. Vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) are extremely important angiogenic factors for vasodilation and neovascularization. We investigated the relationship between single nucleotide polymorphisms (SNPs) of the VEGF and eNOS genes and genetic susceptibility to lumbar IVD degeneration in a young adult Korean population.

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