Interspecific hybrids

Production and genetic characterization of interspecific hybrids among Crambe abyssinica, Crambe hispanica and Crambe kralikii

X. Z. Du, Huang, B. L., Guan, H., Li, Z. Y., and Huang, B. Q., Production and genetic characterization of interspecific hybrids among Crambe abyssinica, Crambe hispanica and Crambe kralikii, vol. 13, pp. 6995-7005, 2014.

In this paper, interspecific crosses among Crambe abyssinica, Crambe hispanica, and Crambe kralikii were reported. In the C. hispanica x C. abyssinica (H x A) cross, 118 F1 hybrids were produced without embryo rescue, while 5 F1 hybrids were obtained with embryo rescue, when C. hispanica was used as the female parent. In the reciprocal cross (A x H), 232 hybrids were obtained without embryo rescue. From more than 1000 C. kralikii flowers pollinated with pollen grains of C.

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

J. Kumchai, Wei, Y. - C., Lee, C. - Y., Chen, F. - C., and Chin, S. - W., Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum, vol. 12, pp. 755-764, 2013.

Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them were confirmed to be true interspecific hybrids. Eighteen and 14 bands from 7 RAPD and 14 ISSR primers, respectively, were polymorphic and present in all five hybrid seedlings and their parents.

Cassava interspecific hybrids with increased protein content and improved amino acid profiles

P. T. C. Gomes and Nassar, N. M. A., Cassava interspecific hybrids with increased protein content and improved amino acid profiles, vol. 12, pp. 1214-1222, 2013.

Cassava (Manihot esculenta) is a principal food for large populations of poor people in the tropics and subtropics. Its edible roots are poor in protein and lack several essential amino acids. Interspecific hybrids may acquire high protein characteristics from wild species. We analyzed 19 hybrids of M. esculenta with its wild relative, M. oligantha, for crude protein, amino acid profile, and total cyanide. Some hybrids produced roots with high protein content of up to 5.7%, while the common cultivar that we examined had just 2.3% crude protein.

Genomic in situ hybridization in plants

G. S. Silva and Souza, M. M., Genomic in situ hybridization in plants, vol. 12. pp. 2953-2965, 2013.

Genomic in situ hybridization (GISH), which is a modification of fluorescent in situ hybridization, has been widely used in the study of plants. It has become one of the most important techniques for molecular cytogenetics. GISH is a technique that allows distinguishing the genomes in a cell. With this technique, it is possible to differentiate the genomes in a hybrid; consequently, this tool has been applied to the study of hybrid lineages, genetic improvement programs, and studies of the evolution of polyploids.

Multivariate analysis of morphological characteristics of two species of passion flower with ornamental potential and of hybrids between them

E. A. Santos, Souza, M. M., Viana, A. P., Almeida, A. A. F., Freitas, J. C. O., and Lawinscky, P. R., Multivariate analysis of morphological characteristics of two species of passion flower with ornamental potential and of hybrids between them, vol. 10, pp. 2457-2471, 2011.

We estimated genetic parameters through multivariate analysis of two species of Passiflora and their hybrids, considered of ornamental potential, based on the morphological characteristics: flower diameter, corona diameter, corona filament size, flower peduncle length, petal length and width, sepal length and width, internode length, stem diameter, leaf length, leaf width (mm), and leaf area (cm2). Five specimens of Passiflora sublanceolata [ex P. palmeri var. sublanceolata], five of P. foetida var.

Amino acid profile in cassava and its interspecific hybrid

N. M. A. Nassar and Sousa, M. V., Amino acid profile in cassava and its interspecific hybrid, vol. 6, pp. 292-297, 2007.

Cassava roots have a low-protein content (0.7-2%). Amino acids such as lysine and methionine are also low, and some research reports have indicated the absence of methionine. The amino acid profiles of a common cassava cultivar and an interspecific hybrid, namely ICB 300, were determined using the computerized amino acid analyzer Hitachi L-8500. The interspecific hybrid has 10 times more lysine and 3 times more methionine than the common cassava cultivar: lysine content was 0.010 g per 100 g in the common cassava cultivar while it reached 0.098 in the interspecific hybrid.

Gene flow between cassava, Manihot esculenta Crantz, and wild relatives

N. M. A. Nassar, Gene flow between cassava, Manihot esculenta Crantz, and wild relatives, vol. 2. pp. 334-347, 2003.

Controlled and natural hybridization between cassava and wild relatives does occur. Barriers within the genus appear to be weak due to recent evolution of the group. All Manihot species examined cytogenetically have a chromosome number of 2n = 36. However, they behave meiotically as diploids. The weak interspecific barriers have led to an extremely heterozygous gene pool that may begin a sequence of hybridization followed by speciation. Introgression from cassava into a number of wild species (M. neusana, M. alutacea, M. reptans and M.

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