Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep
“Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep”, vol. 13, pp. 2645-2653, 2014.
, Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity and accuracy. In this report, we assessed the latest of the non-gel-based methods, high-resolution melting (HRM) curve analysis.